摘要
目的：探讨转化生长因子-β1（TGF-β1）在实验性根尖周病炎症反应和预防中的可能作用。方法：30只Wistar大鼠于上颌1、2磨牙制备根尖周炎动物模型（实验组），下颌1、2磨牙作自身对照组。按开髓后1～10周不同时间点随机分成10组（n=3），采用组织病理学及免疫组织化学方法观察TGF-β1在根尖周炎病变不同阶段中的定位及分布。结果：实验组炎症早期的牙髓（第1～2周）和根尖周（第4～6周）组织中均可见TGF-β1阳性表达，表达细胞多为巨噬细胞、淋巴细胞和成纤维细胞；其染色强度随炎症的发展而改变；牙髓坏死及根尖周炎慢性期（第7周）TGF-β1阳性表达消失。自身对照组下颌第1、2磨牙不同时间点牙髓及根尖部组织均未见TGF-β1阳性染色。结论：TGF-β1在实验性根尖周病变的炎症反应和防御反应中具有一定的调节作用。
Objective To observe the and to discuss its potential function distribution and location of TGF-β1 in different stages of experimental lesions Methods Thirty Wistar rats were established experimental periapical lesions in maxillary first and second molar as experimental group while mandibular molar as control group, and they were divided into ten groups by the time （1, 2, 3, 4, 5, 6, 7, 8, 9, and 10 weeks） after pulp exposure, three rats in every group. Expression of TGF-β1 was detected by histopathology and immunohistochemistry. Results Strong expression of TGF-β1 was observed in acute inflammation of pulp （1st-2nd week） and periapical tissue （4th-6th week）, and idisappeared in pulp aecrosis and in chronic inflammation of periapical tissue （7th week）. The main cells expressing TGF-β1 were macrophage, lymphocyte and fibroblast. Conclusion TGF-β1 has the certain function in inflammation and prevention of experimental periapical lesions.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2006年第5期794-796,F0002,共4页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(970568-2)
关键词
转化生长因子Β
根尖周炎
transforming growth factor beta
periapical periodontitis
