摘要
本研究探讨了乙二胺四乙酸钠（EDTA—Na）对猪卵母细胞的体外成熟和孤雌激活后早期发育的影响。利用屠宰场猪卵巢卵母细胞，在不添加或添加不同浓度EDTA—Na的成熟培养液中体外成熟44～48h，挑选核成熟卵母细胞进行孤雌激活，然后移到不添加或添加不同浓度EDTA—Na的胚胎培养液中进行体外培养168h。结果说明：（1）成熟液中添加适当浓度（25～100μmol／L）EDTA—Na对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用，在EDTA—Na浓度为100μmol／L时，效果最佳；（2）胚胎培养液中添加适当浓度（25～100μmol／L）EDTA—Na，对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用，在EDTA—Na浓度为100μmol／L时，效果最佳；（3）成熟液和胚胎培养液中同时添加100μmol／LEDTA—Na对体外成熟猪卵母细胞孤雌激活后的早期发育具有促进作用；（4）成熟液或／和胚胎培养液中添加不同浓度EDTA—Na，对体外成熟培养后的猪核成熟卵母细胞及孤雌激活后的4-细胞孤雌胚SDS—PAGE电泳的蛋白质表达图谱都无显著影响。
This study was to investigate the effect of EDTA - Na on in vitro maturation of porcine oocytes and early development after parthenogenetically activation . Porcine oocytes from abattoir ovaries were matured in maturation medium whether or not containing different concentrations of EDTA-Na for 44～48 hours. Oocytes with the first polar body were parthenogenetically activated and then cultured in NCSU-23 medium whether or not containing different concentrations of EDTA-Na. The results showed： （1） Supplementation with 25～100μmol/L EDTA-Na in maturation medium was beneficial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro, and when EDTA-Na concentration was 100 μmol/L its effect was the best; （2） Supplementation with 25～100μmol/L EDTA-Na in embryo culture medium was beneficial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro, and when EDTA-Na concentration was 100 μmol/L its effect was the best; （3） Supplementation with 100μmol/L EDTA-Na in maturation and embryo culture media together was benificial to early development of porcine parthenogenetic embryos derived from oocytes matured and artificially activated in vitro; （4） Supplementation with different concentrations of EDTA-Na in maturation medium or/and embryo culture medium had no significant effect on protein expression profiles of SDS-PAGE electrophoresis of porcine oocytes matured in vitro and 4-cell parthenogenetic embryos after artificial activated.
出处
《广西农业生物科学》
CAS
CSCD
2006年第B09期190-190,194,共2页
Journal of Guangxi Agricultural and Biological Science
基金
广西亚热带生物资源保护利用重点实验室-省部共建国家重点实验室培育基地开放课题(SB0505)
广西科学基金项目(桂科青0640002和桂科青0447004)
广西科学研究与技术开发计划项目(桂科攻0537022-01和桂科攻0330004-14)
广州市华粤行仪器有限公司南宁办事处计划内横向项目
关键词
猪
卵母细胞
体外成熟
孤雌激活
胚胎培养
porcine
oocyte
in vitro maturation
parthenogenetic activation
embryo culture
