摘要
利用显微手术切除＋０．２５Ｎ氢氧化钠溶液碱烧伤的方法制备动物实验模型。观察兔眼角膜缘干细胞在角膜上皮损伤愈合中的作用。结果显示：全周角膜缘上皮切除合并角膜上皮缺损组，角膜上皮愈合时间长（２５．８天）。愈合后含有大量新生血管和杯状细胞，呈现结膜上皮表型。半周角膜缘上皮切除合并角膜上皮缺损组，上皮愈合时间缩短（６．３天），含有少量新生血管和杯状细胞。保留角膜缘上皮单纯角膜上皮缺损组，上皮愈合时间最短（３天），无新生血管及杯状细胞，呈角膜上皮表型。结果表明：角膜缘上皮干细胞在角膜上皮损伤愈合中起着极重要的作用，是损伤后角膜上皮再生的源泉。
ornealwoundhealingwasstudiedinarabbitmodelusingsurgicalremovalofepithelialbyoperatingmi-croscopyand0.25NNaOHalkaliburn.Theroleofcorneallimbusstemcelsincornealepithelialwoundheal-ingwasstudied.Theresultsshowedthatthetimeforcornealepithelialwoundhealingwasthelongestinthegroupwithtotalcorneallimbusepithelialdebridementwithcornealepithelialburn(25.8days).Atthistime,thewoundareashowedmorecuppedcelsandcornealvascularization.Thecornealepitheliumhadfeatureofaconjunctivalphenotype.Whenhalfofthecorneallimbusepitheliumwasdebridedalongwithacornealepithe-lialburnwoundhealingwasshortened(6.3days),andlesscornealvasculsrizationandfewercuppedcelswerefound.Woundhealingafteralkaliburnbutnodebridementwastheshortest(3days);therewasnocuppedcelsandnocornealvascularization.Thecornealsurfacehadanormalcornealepithelialphenotype.Thereportshowsthatstemcelsinthecorneallimbusepitheliumhaveanimportantroleincornealepithelialwoundhealingandthattheyaretheultimatesourceofcornealepithelialproliferationaftercornealepithelialwounds.
出处
《眼科研究》
CSCD
1996年第3期149-151,共3页
Chinese Ophthalmic Research
关键词
角膜缘上皮
干细胞
角膜上皮损伤
愈合
corneallimbusepithelial
stemcel
cornealepithelial
epithelialwoundhealing
