摘要
目的探讨缺血-再灌注大鼠胃黏膜毛细血管超微结构的改变。方法SD雄性大鼠随机分为正常对照组(CON)、单纯缺血组(ISC)、缺血再灌注1 h组(I/R1)、24 h组(I/R2)和72 h组(I/R3),用透射电镜观察大鼠胃缺血-再灌注损伤过程中胃黏膜毛细血管超微结构的变化。结果单纯缺血组,毛细血管内皮细胞肿胀,可见核内异染色质向核周聚集;缺血再灌注1 h时,内皮细胞肿胀明显,可见内皮细胞核固缩,管腔闭塞;再灌注24 h后,毛细血管内皮细胞损伤减轻;再灌注72 h后基本恢复正常。结论缺血-再灌注大鼠胃黏膜毛细血管超微结构有变化,内皮细胞呈现细胞凋亡的特征,提示胃黏膜毛细血管形态的改变与胃缺血-再灌注损伤的发生发展密切相关。
Objective To explore the ultrastructural changes of capillaries in the rat gastric mucosa after gastric ischemia - reperfusion. Methods Sprngue- Dawley male rats were randomly divided into 1 control （CON） group, 1 ischemia （ISC） group and 3 ischemia- reperfusion groups （of which the animals in I/R1, I/R2 and I/R3 groups were killed 1, 24 and 72 h after reperfusion, respectively）. The capillary ultrastructural changes of gastric ischemia - repefusion injury were examined under transmission electron microscope. Results In the ISC group, the capillary endothelial cells in the gastric mucosa were swollen, with heterochromatin clustering at the periphery under the nuclear envelope. In the I/R1 group, the endothelial cells were signif- icantly swollen, showing pyknotic nuclei. The capillary lumens were occluded. After repefusion for 24 h, the injury became lightened, and the capillary structures returned to normal after 72 h of reperfusion. Conclusion The findings demonstrate that there are alterations in capillary ultrastructures after gastric ischemia- repefusion and the endothelial changes are of apoptotic characters.
出处
《徐州医学院学报》
CAS
2006年第5期388-391,共4页
Acta Academiae Medicinae Xuzhou
基金
江苏省教育厅自然科学基金资助项目(03KJD310222)
关键词
毛细血管
超微结构
胃缺血-再灌注
大鼠
capilllaries
ultrastructure
gastric ischemia- reperfusion
rat
