摘要
目的研究 ^(188)Re 标记具有 HER-2/neu 癌基因靶向特异性的 Herceptin 免疫磁性纳米微粒及其在小鼠体内的生物学分布,方法利用戊二醛作为交联剂,将人源性单克隆抗体 Herceptin 与化学修饰的磁性纳米微粒进行连接,构建免疫磁性纳米微粒。采用直接标记法将 ^(188)Re 标记到免疫磁性纳米微粒上。采用羰基铼标记法,以 fac-[^(188)Re(CO)_3(H_2O)_3]^+作为放射性标记前体,对表面固载组氨酸的磁性纳米微粒进行标记。分别测定所制备 ^(188)Re 标记物的标记率和体外稳定性及免疫磁性纳米微粒的单克隆抗体免疫活性,并观察 ^(188)Re 标记的磁性纳米微粒及免疫磁性纳米微粒的小鼠体内生物分布。结果经扫描电镜证实免疫磁性纳米微粒的单个粒径大小平均为60 nm,而表面固载组氨酸的磁性纳米微粒的粒径平均为30 nm。^(188)Re 对 Herceptin、免疫磁性纳米做粒及固载组氨酸的磁性纳米微粒的标记率均>90%,在小牛血清中具有良好的体外稳定性,并且磁性纳米微粒上连接的单克隆抗体仍保持较高的免疫活性。小鼠体内分布实验显示 ^(188)Re 标记的磁性纳米微粒及免疫磁性纳米微粒在血液中有较高的放射性分布且血循环时间较长,同时两者在肝内均有较多的摄取。结论 ^(188)Re 标记的磁性纳米微粒及免疫磁性纳米微粒在体外及动物体内较稳定,无明显的 ^(188)Re 脱落。可用于下一步荷瘤裸鼠体内的研究。
Objective To prepare ^188Re labeled monoclonal antibody （Herceptin）-coated magnetic nanoparticles for tumor targeting and to study its biodistribution in mice. Methods Herceptin and histidine were covalently linked to the amine group upon silica-coated magnetic nanoparticles modified by N-[3-（ trimethyoxysilyl） propyl ]-ethylenediamine using glutaraldehyde method. The Heceptin-coated magnetic nanoparticles and Herceptin were radiolabeled with ^188Re by a direct labelling method, whereas the histidine-coated magnetic nanoparticles was radiolabeled with ^188Re using fac-[^188Re（CO）3（H2O）3 ]^＋ as a precursor. The labelling efficiency and immunoreactivity as well as labelling stability were determined. Also, the biodistribution of ^188Re-magnetic and ^188Re-Herceptin-magnetic nanoparticles were observed in mice. Results Herceptin-coated magnetic nanoparticles was characterized by transmission electron microscope （TEM） with diameter about 60 nm, while histidine-coated magnetic nanoparticles about 30 nm. The labeling efficiency for ^188Re-Herceptin, ^188Re-magnetic nanoparticles and ^188Re-Herceptin-magnetic nanoparticles were all 〉 90% and had a better stability in vitro. The immunoreactivity of Herceptin linked to magnetic nanoparticles was still high. The biodistribution in mice was shown that ^188Re-magnetic nanoparticles and ^188Re-Herceptin-magnetic nanoparticles had higher radioactivity levels in blood. Magnetic nanoparticles with diameter of 30 or 60 nm had a long half-life in blood stream and were accumulated in liver. Conclusion The efficiency and stability of labelling Herceptin-coated magnetic nanoparticles and labeling magnetic nanoparticles with raRe are suitable for in vivo study in tumor-bearlng nude mice models.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
2006年第4期231-235,共5页
Chinese Journal of Nuclear Medicine
基金
中国博士后科学基金(2003033345)
关键词
磁性纳米微粒
同位素标记
铼
药代动力学
小鼠
Magnetic nanoparticles
Isotope labeling
Rhenium
Pharmacokinetics
Mice
