摘要
目的建立运用荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测未培养羊水细胞染色体异常的方法。方法对24例未培养羊水细胞进行FISH检测,其中用21号染色体荧光探针检测19例,X/Y染色体探针检测3例,13、18号染色体探针各检测1例。结果24例产前诊断者未培养羊水细胞检测出一例21三体。X/Y染色体探针检测时1例为2个绿色的X信号,2例均为1个红色Y信号和1个绿色X信号。检测结果与培养后羊水细胞检测结果一致,并与外周血染色体核型检测结果相符。结论本研究方法快速、简便、准确可靠,适于临床运用推广。
Objectve： To study the method of the fluorescent in situ hybridization on detection of chromosomes abnormal in uncultured amniotic fluid. Methods： 24 cases of uncultured amniotic fluid were studied, 19 cases were detected by 21 probe chromosome, 3 cases were detected by X/Y probe chromosomes, 2 cases were detecded by 13. 18 probe chromosome respectively. Results： One 21 trisom was observed in 24 cases. 1 case was detected two green X signal and 2 cases were detected one green X signal and one red Y signal with X/Y probes. The results were confirmed by that of cultured amniotic fluid and chromosome analysis of cultured peripheral blood. Conclusion： The method is rapid and accurate, suitable for clinic.
出处
《中国优生与遗传杂志》
2006年第9期33-34,共2页
Chinese Journal of Birth Health & Heredity
关键词
原位杂交
荧光
染色体异常
产前诊断
羊水
In situ hybridization
Fluorescence
Chromosome abnormalities
Prenatal diagnosis
Amniotic fluid
