摘要
在中温（35℃±1℃）厌氧条件下，以葡萄糖为共基质，采用间歇实验方法，研究了2，6-二硝基酚（2，6-DNP）的厌氧产甲烷毒性和厌氧降解动力学。厌氧毒性试验（ATA）以累计产甲烷量和相对活性（RA）为指标，评价了不同浓度2，6-DNP对产甲烷菌的抑制程度；结果表明，2，6-DNP浓度＜20mg／L时，对产甲烷菌没有抑制作用，浓度为40mg／L时产生轻度抑制，浓度为80～120mg／L时产生重度抑制；24h2，6-DNP的75％、50％、25％相对抑制浓度分别为30、70和＞120mg／L。2，6-DNP降解动力学可用Haldane方程来描述，利用非线性拟合求得动力学参数Ks、Rm、Ki分别为179．7mg／L、4．84mg／gVSS·h、206．5mg／L，方差R^2=0．94，拟合效果很好。
Anaerobic toxicity assay （ATA） and degradation kinetics of 2,6-dinitrophenol （2,6-DNP） were investigated in batch culture. The tests were conducted at the temperature of 35℃±1℃ and glucose were used as co-substrate. In anaerobic toxicity assay, accumulative methane production and relative activity （RA） were used to judge the inhibition level of 2,6-DNP on methanogenic bacteria. The results indicated that 2,6-DNP had no inhibition effect on the activity of methanogenic bacteria when the 2,6-DNP concentration was below 20 mg/ L, 2,6-DNP caused slightly inhibition when its concentration was 40 mg/L, and strong inhibition when its concentration was from 80 mg/L to 120 mg/L. The 75% , 50% and 25% relative inhibition concentrations of 2,6- DNP for 24-hour reaction was 30 mg/L, 70 mg/L and 〉 120 mg/L respectively. Haldane equation was proposed to describe the degradation of 2,6-DNP, and the kinetic model parameter Ks, Rm and Ki were estimated by nonlinear curve fit to be 179.7 mg/L, 4.84 mg/ g VSS·h and 206.5 mg/L respectively. The experimental data verification for the model equation is satisfactory. R^2 = 0.94.
出处
《环境污染治理技术与设备》
CSCD
北大核心
2006年第9期31-34,共4页
Techniques and Equipment for Environmental Pollution Control
基金
国家自然科学基金资助项目(40272108)
