摘要
目的研究胶原诱导性关节炎(CIA)大鼠不同时期血清蛋白表达谱,寻找与疾病发生和炎症相关的特异表达蛋白,初步探讨类风湿关节炎(RA)的发病机制。方法以含有19个细胞因子的抗体芯片分别检测CIA大鼠发病高峰期和后期及正常大鼠的血清蛋白表达谱,并以酶联免疫吸附试验法(ELISA)测定血清肿瘤坏死因子(TNF)-α表达,验证微阵列检测结果。结果CIA高峰期和后期持续显著上调的细胞因子有10个,高峰期特异高表达细胞因子有1个。微阵列结果没有显示显著下调的细胞因子。结论CIA疾病不同时期细胞因子表达谱不同,筛选出的特异表达蛋白不仅为进一步研究RA的病理机制提供线索,也为疾病的早期诊断提供分子标志。
Objective This study is undertaken to evaluate the changes of serum cytokine levels in different stages of collagen induced arthritis （CIA） rats, to search for the specific proteins related with rheumatoid arthritis （RA） pathogenesis and inflammation, and to explore the mechanism of RA pathogenesis. Methods Rat cytokine antibody array coated with 19 specific cytokine antibodies was used to examine serum samples at peak and late stage of CIA rats, and were compared to normal cytokine levels. At the same time, ELISA assay for serum TNF-α production was used to verify the array results. Results Among the target cytokines, 10 upregulating cytokines were kept in high expression in different phases of disease, while 1 showed significant change only at the peak of disease. There was no downregulating cytokines in the results. Serum TNF-α assay results were consistent to the array results. Conclusion Cytokines show different expression in CIA at different stages, and specific cytokines can be used as the candidates to further study of the RA pathogenesis. This study also provides molecular makers for early diagnosis.
出处
《中华风湿病学杂志》
CAS
CSCD
2006年第9期538-541,i0001,共5页
Chinese Journal of Rheumatology
基金
中国博士后科学基金(2005037220)
