摘要
目的研究125I-Genistein在荷人乳腺癌裸鼠体内的生物学分布情况,探讨Genistein用于治疗人乳腺癌的可行性。方法Iodogen法标记Genistein;建立荷人乳腺癌裸鼠移植瘤模型;测定125I-Genistein在荷瘤裸鼠体内的放射性分布,计算肿瘤(T)与非肿瘤组织(NT)的放射性比值。结果125I-Genistein比活度为336KBq/μg,放射性化学纯度为98.72%。尾静脉给药30min后肿瘤组织的放射性达到高峰,但在各观察时相点肿瘤(T)放射性均显著低于血液、肝脏与肾脏(NT)(P<0.01);肾脏放射性明显高于肠道,30min后高于除血液外的其他受检组织;肠和脾脏的放射性始终处于极低水平。结论Genistein用于人乳腺癌的治疗需要进一步研究,以提高其在肿瘤组织中的分布。
Objective To study the biodistribution of ^125I Genistein in nude mice bearing human breast cancer, and to explore the possibility of genistein to treat human breast cancer intravenously. Methods Genistein was labeled with Na ^125I by using the Iodogen method, A nude mice model which bearing MCF-7 cells derived from human broast cancer was established, The biodistribu tion of ^125I Genistein was detected in nude mice bearing human breast cancer from 10min to 240min after intravenous injection. Resuits The specific activity of ^125I-Genistein was 336KBq/μg. and radiochemical purity of ^125I-Genistein was 98.72%. The biodis tribution experiment showed that the peak time of ^125I-Genistein concentrating in the tumor was about 30min alter injection, The radioactivity in tumor was lower than that in blood.liver and kidneys（P〈0,01）, The radioactivity in kidneys was higher than that in intestines and there was low level of radioactive distribution in lungs and spleen at all phases. Conclusion The rcsuhs of present study suggest to enhace the tumor intake of genistein for breast cancer therapy.
出处
《重庆医学》
CAS
CSCD
2006年第18期1666-1667,1670,共3页
Chongqing medicine
基金
重庆市院士专项基金资助项目(2004BC5006)
