摘要
目的：研究生长激素1（growth hormone1，GHl）基因T1663A多态性与结直肠癌易感性的关系。方法；在江苏省进行了一个病例一对照研究（结直肠癌患者315例，人群对照439名），调查研究对象的生活习惯，抽取静脉血，提取白细胞DNA，采用PCRRFLP检测研究对象的GHlT1663A基因型。结果：1）GHlT／T、T／A和A／A基因型分布频度在结直肠癌组分别为42．2％、46．7％和11．1％，对照组分别为38．1％、45．9％和16．0％，两组差异无统计学意义，拍“-3．907，P-0．142。但在调整性别、年龄、吸烟和饮酒习惯后，A／A基因型者与T／T基因型者相比，发生结直肠癌的危险性显著降低（0R-0．88，95％CI：0．78～0．99，P=0．0287）。2）多因素分析结果显示，饮酒者患结直肠癌的危险性显著增高（0R=1．96，95％CI：1．34～2．86，P=0．0005），A／A基因型与降低结直肠癌的危险性有关，而吸烟与增加或降低结直肠癌的危险性无显著相关。3）GHl基因多态与吸烟、饮酒相互作用的分层分析发现，在不吸烟者中，GHlA／A基因型者与T等位基因型者相比，发生结直肠癌的危险性显著降低（性剐和年龄调整OR=0．50，95％CI：0．27～0．93）；在不饮酒者中，GHlA／A基因型者发生结直肠癌的调整OR为0．56（95％CI：0．32～0．99）。结论：T1663A GHl基因的A／A基因型可降低结直肠癌易感性，特别是在不吸烟和不饮酒者中。
OBJECTIVE： To study the relationship between genetic polymorphisms of growth hormone 1 （GH1） T1663A and the susceptibility of colorectal cancer （CRC）. METHODS： A case control study was conducted with 315 patients with CRC and 439 population based controls in Jiangsu Province, China. The epidemiological data were collected, and DNAs of peripheral blood leukocytes were obtained from all of the subjects. GH1 genotypes were detected by PCR-RFLP. RE- SULTS： 1） The frequencies of the GH1 T/T, T/A and A/A genotypes were 42.2%, 46. 7% and 11.1% in colorectal cancer cases, and 38. 1~, 45.9% and 16. 0% in controls, respectively. Compared with individuals who had GH1 T/T genotype, individuals who had GH1 A/A genotype were at a decreased risk of developing colorectal cancer （sex-, age-, smoking and alcohol drinking-adjusted OR=0. 88, 95%CI： 0. 78--0. 99, P=0. 029）. 2） Smoking was not associated with the risk of colorectal cancer, whereas alcohol drinking was associated with the increased risk of colorectal cancer （sex-, age-, smoking and GH1 genotype-adjusted OR= 1.96, 95%CI： 1.34--2.86）. 3） Among non-smokers, individuals who had GH1 A/A genotype were at a decreased risk of developing colorectal cancer （sex-, age-adjusted OR= 0. 50, 95 %CI： 0. 27--0. 93） compared with individuals who had GH1 T allele. Among non-drinkers, individuals who had GH1 A/A genotype also were at a decreased risk of developing colorectal cancer （sex, age-adjusted OR = 0. 56, 95 % Cl ： 0. 32-- 0. 99） compared with individuals who had GH1 T allele. CONCLUSION： The results show that the GH1 gene T1663A polymorphism can influence suscep tibility of colorectal cancer, particularly in non-smokers or non-drinkers.
出处
《中华肿瘤防治杂志》
CAS
2006年第15期1134-1137,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
日本文科省国际学术研究癌症特别研究经费(08042015)
中国国家自然科学基金重大国际(地区)合作研究项目资助(30320140461)
关键词
结直肠肿瘤/遗传学
生长激素
多态性
限制性片段长度
病例对照研究
colorectal neoplasms/genetics
growth hormone
polymorphism, rest ＂iction fragment lengt h
case-control study
