摘要
利用脂肪酶Novozym435在正丁醇体系中催化酯交换反应，对（R，S）．扁桃酸乙酯进行了动力学拆分，考察了系统初始加水量，反应温度，振荡速度，底物浓度等因素对脂肪酶催化活性和对映体选择性的影响．研究结果表明，最适初始加水量为0．4％；在20℃-60℃范围内，酶催化活性随温度升高而增加，酶选择性随温度先升高后下降，最适温度45℃；底物扁桃酸乙酯浓度达5000mmol／L时，未观察到底物抑制现象，反应初速度为2．78mmol·L^-1·min^-1．
Novozym 435 catalyzed enantioselective transesterification was employed for kinetic resolution of racemic ethyl mandelate in n-butanol. Effects of various process parameters such as initial content of water, reaction temperature, shaking speed and substrate concentration on the reaction were studied. The optimum initial content of water was 0.4%. The reaction rate was increased by increasing reaction temperature from 20 ℃ to 60 ℃, while the best enantioselectivity was observed at 45℃ Up to a substrate concentration of 5000 mmol/L, the initial reaction rate was 2.78 mmol·L^- 1·min ^- 1, with no obvious substrate inhibition being found.
出处
《分子催化》
EI
CAS
CSCD
北大核心
2006年第4期351-354,共4页
Journal of Molecular Catalysis(China)
基金
国家教育部重点项目(编号:JA0213903062)
福建省发展与改革委员会项目(闽计46号)
福建省自然科学基金(编号:C0510006)
