摘要
目的研究人参皂苷Rg_3与羟丙基-β-环糊精(HP-β-CD)在水溶液中的包合作用、包合比及包合过程中的热力学参数变化。方法采用相溶解度法、薄层色谱法、红外光谱法、核磁共振法测定人参皂苷Rg_3与HP-β-CD在水溶液中包合作用、包合比及包合过程中热力学参数变化。结果在水溶液中人参皂苷Rg_3与HP-β-CD均可形成1∶1摩尔比可溶性包合物;人参皂苷Rg3的苷元部分可能嵌入HP-β-CD分子的疏水性空穴中,两个葡萄糖裸露在环糊精腔外。对包合物进行了鉴定和确证,表明人参皂苷Rg_3与羟丙基-β-环糊精已经形成包合物。人参皂苷Rg_3与HP-β-CD在水溶液中的包合过程可自发进行(△G<0),且为放热反应(△H<0),同时也是熵减过程(△S<0)。结论人参皂苷Rg_3与HP-β-CD在水溶液中可自发形成1∶1摩尔比可溶性包合物,从而增加溶解度。选择适宜的包合温度将有利于包合作用的进行。
Objective To investigate the inclusion of ginsenoside Rg3 with hydroxylpropyl-β-cyclodextrin （HP-β-CD） in aqueous solutions, inclusion molar ratio of host and guest molecules, and change of thermodynamic parameters during the inclusion process. Methods The measurements of the inclusion mechanism, inclusion molar of the host and guest molecules, and change of thermodynamic parameters were carried out by the following methods： phase solubility, IR, NMR, and TLC methods, respectively. Results The results indicated that the inclusion compound of ginsenoside Rg3-HP-β-CD was formed. The content analysis of the inclusion compound showed that the inclusion ratio of ginsenoside Rg3-HP-β-CD was 1：1. The change in the thermodynamic parameters suggested that the inclusion could proceed （△G〈0） spontaneously along with the heat release （△H〈0） and the entropy decrease （△S〈0）. Conclusion A molar ratio inclusion complex （1：1） of ginsenoside Rg3 with HP-β-CD in aqueous solution could be formed spontaneously and, hence the solubility of ginsenoside Rg3 in aqueous solution increased. Appropriate temperature probably favors the progress of inclusion procedure.
出处
《中草药》
CAS
CSCD
北大核心
2006年第9期1328-1331,共4页
Chinese Traditional and Herbal Drugs
关键词
人参皂苷RG3
羟丙基-Β-环糊精
溶解度
包合比
热力学参数
ginsenoside Rg3
hydroxypropyl-β-cyclodextrin （HP-β-CD）
solubility
inclusion ratio
thermodynamic parameter
