摘要
目的 采用手术方法结扎门静脉分支，观察对侧肝脏的再生状态。方法 健康Wistar雄性大鼠60只，随机均分成结扎组和假结扎组，在乙醚麻醉下行门静脉左支结扎和假结扎手术，分别在术后第1、2、3、7及14d用乙醚麻醉动物，心脏采血检测血清ALT值，取出肝脏后称重量；在光镜下观察肝组织的病理变化，并计数肝细胞核分裂指数；采用免疫组化的方法计数增殖细胞核抗原（PCNA）指数；在电镜下观察肝细胞超微结构变化。结果 ①血清ALT值结扎组与假结扎组比较在术后第1d升高（P〈0．01），但在第2d开始恢复正常；②肝脏重量两组间差异无统计学意义（P〉0．05）；③肝细胞核分裂指数结扎组与假结扎组比较，术后第1～3d明显升高，第2d达高峰，第3d有所下降，但仍高于假结扎组（P〈0．01），以后逐渐恢复正常；④PCNA阳性细胞计数结扎组与假结扎组比较，术后第1～3d明显增多，第2d达高峰，第3d有所减少，但仍高于假结扎组（P〈0．01），以后逐渐恢复正常。结论 ①大鼠门静脉左支结扎后，引起未结扎侧肝细胞的活跃再生，再生后的肝脏可恢复原来的重量；②大鼠75％肝叶的门静脉分支结扎不影响肝功能，是安全可行的；③大鼠门静脉分支结扎可以作为研究肝脏再生动物模型的方法。
Objective To study liver regeneration of the non ligated liver lobes following portal branch ligation （PBL）. Methods Sixty male Wistar rats were randomly divided into PBL group and sham operation （SO） group. Under ether anesthesia, the rats were subjected to PBL and sham operation, respectively. The animals were sacrificed on the 1st, 2nd, 3rd, 7th and 14th day respectively. The blood sample was collected from heart and the livers were harvested to determine serum alanine aminotransferase （ALT） levelg and total liver weight, respectively. The hepatic histopathology was studied through light microscopy. The number of liver cell nuclear mitosis index was counted. The number of proliferative cell nuclear antigen （PCNA） index was counted by immunohistochemistry. The hepatic ultrastructural changes were studied under electron microscope. Results ①Elevated serum AI.T level was observed in the first postoperative day in PBL group compared with SO group （P〈0.01）, but began to recover in the second day. ②No significant total liver weight change in PBL group and SO group were found. ③Liver cell nuclear mitosis index and PCNA index were markedly increased in PBI. group compared with SO group in day 1- 3 postoperative day （P〈0.01）. It reached the peak in the second day and decreased slightly in the 3rd day, but still higher than SO group, then gradually return to normal lately. Conclusion The ligation of left portal branch can induce active regeneration of hepatic cell of non-ligated liver lobes in rats. The regeneration of non-ligated liver lobes may restore previous total liver weight. The ligation of 75 % portal branch does not affect liver function and may be safely performed. The portal branch ligation in rats may be used as an animal model in study of liver regeneration.
出处
《中国普外基础与临床杂志》
CAS
2006年第5期550-554,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
结扎
门静脉分支
肝再生
Ligation Portal branch Liver regeneration
