摘要
目的研究早期自然流产患者绒毛肿瘤转移抑制基因KiSS-1与基质金属蛋白酶(MMP-9、MMP-2)mRNA及蛋白的表达,探讨其与自然流产的关系。方法采用半定量RT-PCR、免疫印迹法检测30例正常早期妊娠和30例早期自然流产患者绒毛KiSS-1、MMP-9和MMP-2的mRNA及蛋白水平,用明胶酶谱分析法检测上述样本孵育液MMP-9、MMP-2的酶活性。结果(1)自然流产患者绒毛组织中MMP-9及MMP-2mRNA表达水平低于正常早期妊娠组(P<0.05),KiSS-1mRNA的表达高于正常早期妊娠组差异有统计学意义(P<0.05);(2)Westernblotting分析显示自然流产患者绒毛MMP-9、MMP-2蛋白表达低于正常早期妊娠差异有统计学意义(P<0.05),KiSS-1蛋白表达高于正常早期妊娠差异有统计学意义(P<0.05);(3)明胶酶谱分析结果显示,自然流产患者绒毛MMP-9、MMP-2酶活性低于正常早期妊娠者差异有统计学意义(P<0.05)。结论自然流产患者绒毛促浸润基因MMP-9、MMP-2mRNA及其蛋白表达水平降低,同时抑制浸润基因KiSS-1mRNA及其蛋白表达增加,造成滋养细胞浸润能力下降,可能与自然流产的发生有关。
Objective：To investigate the mRNA and protein expression of metastasis suppressor gene KiSS-1, matrix metalloproteinase-9 （MMP-9） and MMP-2 in villi of first trimester spontaneous abortion. Methods： Expression of KISS-1, MMP-9 and MMP-2 mRNA and protein in villi of first trimester spontaneous abortion （ abortion group, n = 30） and normal first trimester preg.nant women（ control group,n = 30） was detected by Semi-quantitative RT-PCR , Western blotting and gelatin zymography. Results： （ 1 ） Expression of MMP-9 and MMP-2 mRNA was significantly lower in villi of abortion group compared with that of control group （P 〈 0. 05 ）. Expression of KiSS-lmRNA increased significantly in villi of abortion group （P 〈 0. 05 ） ; （2） Western blotting analysis demonstrated that expression of MMP-9 and MMP-2 protein in villi of abortion group was significantly lower than that of control group （ P 〈 0. 05 ）. Expression of KiSS-1 protein in villi of abortion group was significantly higher than normal（ P 〈0. 05 ） ; （3） Gelatin zymographic analysis showed enzyme activity of MMP-9 and MMP-2 in harvested villi culture medium of abortion group was significantly lower than that of control group （P 〈 0.05 ）. Conclusion ：This study showed that MMP-9, MMP-2 and KiSS-1 may play an important role in the invasion of human placenta trophoblast. It may correlate with occurrence of first trimester spontaneous abortion.
出处
《现代妇产科进展》
CSCD
北大核心
2006年第8期603-607,共5页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金资助项目(30271362)
