摘要
Aim To investigate the survival rate and the level of HaCaT cells damage with ultraviolet B（UVB） radiation at various doses,and observe the protective effects of ginsenoside Rg1 and Rb1 in vitro.Methods MTT assay was employed to analyze the cell survival rate after UVB radiation of 30,60,90 and 120 mJ·cm-2.The damage of nucleolus and the protective effects of ginsenoside Rg1 and Rb1 were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay（SCGE）.Results It was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ·cm-2 to 120 mJ·cm-2.At the dose of 20 μg·mL-1,obvious protective effect of ginsenoside Rg1 and Rb1 can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage.Conclusion UVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rg1 and Rb1 can relief the damage.
Aim To investigate the survival rate and the level of HaCaT cells damage with ultraviolet B （UVB） radiation at various doses, and observe the protective effects of ginsenoside Rgl and Rbl in vitro. Methods MTT assay was employed to analyze the cell survival rate after UVB radiation of 30, 60, 90 and 120 mJ·cm^-2 The damage of nucleolus and the protective effects of ginsenoside Rgl and Rbl were scanned by Hoechst 33258 staining and single cell gel electrophoresis assay （SCGE）. Results It was found that the cell survival rate decreased gradually and the damage of nucleolus aggravated as the radiation dose increased from 30 mJ ·cm^-2 to 120 mJ · cm^-2 At the dose of 20 μg ·mL^-1, obvious protective effect of ginsenoside Rgl and Rbl can be observed against UVB radiation-induced HaCaT cells growth inhibition and nucleolus damage. Conclusion UVB radiation inhibits HaCaT human keratinocytes growth and ginsenoside Rgl and Rbl can relief the damage.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第9期905-908,共4页
Acta Pharmaceutica Sinica
基金
四川省科委基金资助项目(02SY029-137)
