摘要
目的建立HPLC法同时测定复方黄芪注射液中人参皂苷Re、Rg1的含量。方法采用HPLC法，色谱柱为Diamonsil TMCJ8柱（4．6mm×250mm，5μm），流动相为乙腈-50mmol·L^-1磷酸二氢钾缓冲溶液（体积比为20：80），柱温为25℃，检测波长为203nm，流速为1．0mL·min^-1。结果复方黄芪注射液中人参皂苷Re、Rg1与其他成分分离良好。人参皂苷Re的质量浓度在25～500mg·L^-1内（r=0．9998）、Rg1的质量浓度在25～300mg·L^-1（r=0．9998）内与峰面积线性关系良好，人参皂苷Re、Rg1的回收率分别为100．2％和99．8％，RSD分别为1．4％和2．0％。结论方法准确、重现性好，可用于复方黄芪注射液的质量控制。
Objective To determine the contents of the ginsenoside Re and Rgl in the compound Huangqi （traditional Chinese medicines） injection. Methods The DiamonsilTM C18 （4.6 mm × 250 ram, 5μm） column was used with a mobile phase of acetonitrile-50 mmol.L^-1 potassium dihydrogen phosphate buffer （ V ： V = 20 ： 80）, the wavelength of the detector was set at 203 nm. Results The linear ranges of the ginsenoside Re and Rgl were 25 - 500 mg.L^- 1 （ r = 0. 999 8） and 25 - 300 mg. L^- 1 （ r = 0. 999 8） respectively. The average recoveries of the ginsenoside Re and Rgl were 100.2 % and 99.8 %, the RSDs were 1.4 % and 2.0 %, respectively. Conclusions The method is simple and accurate with a good reproducibility and can be used as a quality control method for compound Huangqi injection.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2006年第9期577-579,606,共4页
Journal of Shenyang Pharmaceutical University
