摘要
经大肠杆菌E．coli K12D31诱导后的家蚕幼虫，其免疫血淋巴经CM-Sepharose CL-6B离子交换层析、Sephadex G-100凝胶过滤层析及HPLC分离后，得到2种抗菌蛋白，经液相色谱-电喷雾质谱（ESI-MS）分析鉴定，纯化的2种抗菌蛋白分别是家蚕抗菌肽eeeropin D和溶菌酶lysozyme酸性电泳（A-PAGE）测活免疫血淋巴得到抗菌蛋白活性谱：cecropin D在8h无显著表达，12h有较强抗菌活性，30h达到最高，以后逐渐降低；lysozyme在8h后检测到表达，12h到达高峰，之后逐渐下降，48h的血淋巴中未检测到明显的表达．研究认为抗菌蛋白lysozyme和cecropin D是家蚕幼虫感染细菌8h后大量表达的抗菌蛋白，主要参与细菌感染12～30h的血淋巴对细菌的清除，是参与家蚕幼虫抗菌免疫的重要蛋白．
Injection of E. coli K12 D31 into Chinese silkworm larave, Bombyx mori, the antibacterial activity is detected in the hemolymph. After being separated by CM-Sepharose C1 -6B, sephadex G - 100 chromatography and HPLC, two antibacterial proteins are identified. These two proteins are antibacterial peptide cecropin D and lysozyme identified by ESI - MS. Testing antibacterial activity by A - PAGE analyses reveals that both cecropin D and lysozyme are not detected before 8h after E. coli injection. Lysozyme reaches to the maximal level at 12 h and then gradually decreases. Cecropin D is detected in induced hemolymph at 12 h and 30 h. These two antibacterial proteins are almost not detectable at 48 h. Results show that cecropin D and lysozyme play a vital role in antibacterial immunity of silkworm at 12 h -36 h.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期103-107,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
南京师范大学校青年基金资助项目(2001SWXXQNBQ12)
