摘要
分别用不同浓度的氯化锶联合细胞松弛素、钙离子载体A23187和乙醇分别与6-二甲氨基嘌呤和细胞松弛素B刺激小鼠卵母细胞,比较卵母细胞的激活率和体外发育情况。结果表明,乙醇+6-DMAP+CB和A23187+6-DMAP+CB激活获得的激活率、桑囊胚率较高。
The objective of present study was to compare parthenogehic development of mouse oocytes through the treatment of the different medical methods with different concentration SrCl2 and 5μmol/L cytochalasin B （CB）and either 10 % alcohol or 5μmol/L calciumion A23187, and either 2 mM 6-dimethylaminopurine （6-DMAP） or 6-DMAP＋5 μmol/L cytochalasin B（ CB）.In experiment, Oocytes were activated with 1.5, 5.0, 10.0 and 15.0 mmol/L SrCl2 and then incubated with 5 μmol/L CB. The results showed that Alchol-6-DMAP＋CB had a higher rate of morula and blastoeyst rates and activation rates than alchol-6-DMAP and alchol- CB （P〈O.05）, A 23187-6-DMAP ＋CB was also higher than A 23187-6- DMAPand A 23187-CB （P〈0.05）.
出处
《安徽农业科学》
CAS
北大核心
2006年第16期4008-4009,4037,共3页
Journal of Anhui Agricultural Sciences
基金
青岛市科技将才资助。
关键词
小鼠
卵母细胞
孤雌激活
化学激活剂
Mouse
Oocytes
Parthenogenesis
Chemical activation reagent
