摘要
伤寒V i多糖与白喉类毒素(DT)蛋白以己二酰肼(ADH)作连接臂,在碳二亚胺(EDAC)的作用下结合成V i-DT结合物,进行抗原性、免疫原性和动物安全性试验。结果显示,3批结合物抗体阳转率均为100%,加强免疫一针后GMT值明显升高(p<0.001),免疫剂量以0.75μg/只抗体增长最为明显,甚至以低剂量0.375μg/只仍能获得较高的抗体滴度,多项动物试验证明该结合物是安全的。显示V i-DT结合物有很好的动物安全性和免疫原性,为临床试验提供实验室依据。
The Vi capsular polysaeeharide of Salmonella typhi was bound to diphtheria toxoid （DT） with adipie acid dihydrazide （ADH） as a linker, and EDAC used as a eondensating agent. The reaction mixture was passed through a Sephaeryl S-1000 column （ 2.6 × 90 cm） along with a equilibrated saline, the pooled fractions from void volume were conjugates. The conjugates were analyzed and injected in mice and Guinea pigs to evaluate the immunogenieity and animal safety. Vi-DT conjugates still maintain the antigenieity of Vi and elieite high levels anti-Vi IgG in mice. The ehareetersties of conjugates are in eortformation with the requirements for toxicity tests, and they were safe in animal test.
出处
《微生物学免疫学进展》
2006年第3期5-8,共4页
Progress In Microbiology and Immunology
关键词
结合疫苗
伤寒
安全性
免疫原性
Conjugate vaccine
Typhoid fever
Safety
Immunogenieity
