摘要
按常规饲养条件饲养AA雄性肉鸡120羽,30日龄时随机分为试验组(T组)和对照组(C组)。T组肉鸡静脉注射L-AG(40 mg/kg),C组肉鸡静脉注射生理盐水。分别在注射L-AG和生理盐水1、2、4 h后测定平均肺动脉压(mPAP)、诱导型一氧化氮合酶(iNOS)活性、原生型一氧化氮合酶(cNOS)活性、一氧化氮(NO)含量、红细胞压积(PCV)、电解质浓度、pH、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性和丙二醛(MDA)含量。结果显示:(1)试验组mPAP、NO含量和iNOS活性均显著或极显著低于对照组(P<0.05或P<0.01);(2)PCV和Ca2+在给药后2h和4 h与对照组差异显著(P<0.05);(3)K+浓度在给药1 h和2 h后显著或极显著低于对照组(P<0.05或P<0.01)。结果表明,L-AG通过抑制iNOS活性,引起肉鸡肺动脉压的升高,从而推测iNOS具有调节肉鸡体内mPAP的作用并与肉鸡肺动脉高压综合征发生有着密切的联系。
This experiment was conducted to evaluate the effect of L-AG on the pulmonary hypertension and other concerned index in broilers. One hundred and twenty 1-day-old commercial male broilers were raised according to AA feeding condition until 30 days and then the broilers were divided into a experimental （T） group and a control （C） group. Group T and Group C were given L-AG at dose of 40 mg/kg and physiological saline through intravenous injection, respecitively. The mPAP, inducible oxide synthase （iNOS） activity, constitutive oxide synthase （cNOS） activity, nitric oxide （NO） level, PCV, electrolyte level, pH, s uperoxide dismutase （SOD）, catalase （CAT）, malondialdehyde （MDA） were measured at 1,2,4 hours after administration of L-AG and physiological saline. Results were as follow： （1）From 1 to 4 hours,the mPAP,the NO level and the iNOS activities of group T were significant higher than that of group C（P〈0.05 or P〈0.01 ） ; （2）Compared with group C, PCV and Ca^2＋ of group T were significant difference within 2-4 hours after administrating the dose （P〈0. 05）; （3）Compared with group C,K^＋ concentration was significant lower within 1-2 hours after administering the dose（P〈0. 05 or P〈0.01 ）. It is suggested that the L-AG can induce pH by inhibiting the serum iNOS activity,therefore ,iNOS has an effect on adjusting the mPAP of broilers and my play an important role on the mechanism of PHS.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第5期547-550,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30270997)
