摘要
将体质量100～120g的雄性SD大鼠258只随机分为7组。Ⅰ组，0．3mg／kg富硒麦芽硒组；Ⅱ组，1mg／kg富硒麦芽硒组；Ⅲ组，3mg／kg富硒麦芽硒组；Ⅳ组，3mg／kg亚硒酸钠硒组；Ⅴ组，诱癌阳性对照组；Ⅵ组，3mg／kg亚硒酸钠硒不诱癌组；Ⅶ组，阴性对照组，不加硒也不诱癌。Ⅰ～Ⅴ组，饮水中添加二乙基亚硝胺（DENA，100mg／L）以诱癌。每2周眼眶采血1次，测定血浆丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、总胆红素（TBIL）、白蛋白（ALB）、葡萄糖和血清钙含量、全血GSH-Px活性等。结果发现，富硒麦芽硒比亚硒酸钠硒能更有效地抑制DENA所致大鼠肝损伤，提高大鼠全血GSH-Px活性，并以1mg／kg添加刺量效果较佳；DENA所致大鼠肝肿瘤初期即伴随着明显的低血糖症。结果表明：富硒麦芽比亚硒酸钠能更有效抑制DENA所致的初期肝损伤，保护肝脏，提高抗氧化能力。
Two hundred and fifty eight SD rats weighted 100-120 g were divided randomly into 7 groups. The selenium contents in feed of group Ⅰ , Ⅱ and Ⅲ were 0.3,1.0,3.0 mg/kg in Se-enriched malt respectively. 3 mg/kg selenium in sodium selenite was supplemented to the diets of groups Ⅳ and Ⅵ. Rats in group Ⅴ and group Ⅶ received 0. 1 mg/kg selenium in sodium selenite only and were used as the positive control group and negative control group respectively. Rats in groups Ⅰ - Ⅴ were treated with diethylnitrosamine （100 mg/L） to induce liver cancer. The liver function indices, named as ALT, ALP, TBIL and ALB in plasma,glucose content in plasma,calcium concentration in serum,glutathione peroxidase （GSH-Px） in whole blood were measured in every other week. The data showed that effect of selenium in Se-enriched malt excelled sodium selenite in the aspects of raising GSH-Px activity and deadening the early lesion induced by diethylnitrosamine. In term of this aspect,malt-selenium with the concentration of 1 mg/kg in diet achieved a preferable effect. Hypoglycemia was evident in early progress of hepatocarcinogenesis in rats induced by diethylnitrosamine. In conclusion,the effect of selenium in Se-enriched malt on stimulating the antioxidative function and suppressing the lesion induced by diethylnitrosamine was better than that of sodium selenite.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第5期512-515,共4页
Chinese Journal of Veterinary Science
基金
高校博士点基金(20020307022)
国家"十五"科技攻关项目(2002BA574A-10-1-6)
江苏省"九五"农业重点攻关项目(Q9837)
南京农业大学青年科技创新基金(KJ05015)
关键词
富硒麦芽
肿瘤
大鼠
血糖
血钙
GSH-PX
selenium-enriched malt
tumor
rats
plasma glucose
serum calcium
GSH-Px
