摘要
目的 建立大鼠28天染毒的特异性体液免疫毒性模型，说明溶血空斑试验和血清溶血素试验用于评升大鼠特异性体液免疫毒性的可行性。方法 雄性Wistar大鼠40只，体重180～200g，随机分为对照组和5，10，20mg／kg环磷酰胺（已知的免疫抑制剂）染毒组，每组10只大鼠，各剂量组每日灌胃染毒1次，连续28d，对照组给予生理盐水；染毒第24天，大鼠腹腔注射1ml 10％绵羊红细胞进行免疫；染毒结束后24h，断头处死大鼠，取脾脏，制备脾细胞悬液，进行溶血空斑测定；收集血液，制备血清，进行血清溶血素试验。结果5，10和20mg／kg环磷酰胺染毒组大鼠的溶血空斑数（分别为159．6±91．8，93．6±14．8，39．8±11．0）及血清溶血素水平（分别为125．6±38．5，36．4±5．9，1．5±0．3）低于对照组（溶血空斑数为355．0±96．3，血清溶血素水平为283．1±96．3），差异有显著性（P〈0．05）。结论 溶血空斑试验和血清溶血素试验两种方法用于评价大鼠特异性体液免疫功能具有可行性，可为我国化学品免疫毒性评价方法的建立提供参考。
Objective To establish a specific humoral immunotoxicity model in rats orally exposed to cyclophosphamide for 28 days and to validate the assays of antibody plaque-forming cells （PFCs） and serum hemolysin concentration （HC50）. Methods 40 Male Wistar rats with body weight of 180 ～ 220 g were randomly divided into control group and groups of 5, 10, 20 mg/kg cyclophosphamide （known as suppressive immunotoxicity） with 10 rats in each group. The rats were orally exposed to cyclophosphamide once a day successively for 28 days. The rats in the control group received normal saline. At the 24^th days of exposure, the rats received 1 ml of 10% sheep red blood cells （SRBCs） by i.p. injection. 24 hours after the end of 28 days exposure, the rats were decapitated and their spleens were excised. The spleen single cell suspension was prepared for PFCs assay; at the same time, the blood was collected and serum was prepared for HC50 assay. Results The PFCs in groups of 5, 10 and 20 mg/kg cyclophosphamide （respectively 159.6±91.8, 93.6±14. 8, 39.8±11, 0） and HC50 in groups of 5, 10 and 20 mg/kg cyclophosphamide （respectively 125.6 ±38.5, 36.4 ± 5.9, 1.5 ± 0.3） decreased significantly compared with those of the control group （PFCs 355.0±96.3 and HC50 283.1±96.3）, P〈0.05. Conclusions The results validate the PFCs assay and the HC50 assay, which may provide a reference for establishing the immunotoxicity evaluation methods in China.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2006年第5期280-282,共3页
Industrial Health and Occupational Diseases
关键词
环磷酰胺
溶血空斑
溶血素
大鼠
Cyclophosphamide
Plaque-forming cells
Serum hemolysin eoneentration
Rats
