摘要
目的探讨白细胞介素-1(IL-1),尤其是白细胞介素-1β(IL-1β)和白细胞介素-1受体拮抗剂(IL-1RA)基因多态性及IL-1β血清含量与东北地区汉族多发性硬化(M S)的关系。方法多聚酶链反应(PCR)对54例M S患者的血清进行IL-1β和IL-1RA基因多态性检测;酶联免疫吸附实验检测血清中IL-1β含量。结果IL-1β和IL-1RA基因型分布及等位基因频率在M S和对照组间无显著性差异(P>0.05)。IL-1β血清含量M S组比对照组显著增高(P<0.05)。结论IL-1β和IL-1RA基因多态性与M S易感性可能无相关性。IL-1β可能在M S发病中起到促进作用,参与启动炎症过程和组织损伤。
Objective To implore the relationship between interleukin-1,especially interleukin-1β（IL-1β） and interleukin-1 receptor antagonist （IL-1RA）gene polymorphism and the serum level of IL-1β and the multiple sclerosis（MS）patients in the Northeast of China. Methods DNA of white blood cells from 54 subjects with MS and 60 healthy controls was extracted. Polymorphisms in IL-1β and IL-1RA were analyzed with PCR in order to find the difference of the genotype distribution and the allele frequency between the MS patients and the healthy controls. Results The genotype（CC,CT,TT）distribution of IL-1β was 44.4%,22.2%,and 33.4% in patients with MS and 46.7%,23.3% ,and 30.0% in controls（P〉0.05） ;while for the IL-1RA（Ⅰ/Ⅰ,Ⅰ/Ⅱ,Ⅱ/Ⅱ,Ⅰ/Ⅳ） was 81.5%,7.4%, 5.6%,5.5% in MS patients and 80.0%,8.3%,5.0%,6.7% in controls（P〉0.05）. The allele frequency of IL-1β in patients with MS were C 61.1% ,T 38.9% and 60.0% ,40.0% in controls ;white for the IL- 1RA（Ⅰ,Ⅱ,Ⅳ） were 85.2%,9.3%,5.5% in MS patients and 83.3%,10.0%,6.7% in controls. （P〉0.05）. The serum production of IL-1β was 146.83pg/ml in MS patients and was 96.23pg/ml in controls（P%0.05）. Conclusion The IL-1β and IL-1RA polymorphisms were not significantly associated with the susceptibility of MS,while IL-1β maybe play an active role in the MS and be involved in the start of inflammation progression and tissue damage.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2006年第3期344-346,共3页
Journal of Apoplexy and Nervous Diseases
关键词
IL-1Β
IL-1RA
多发性硬化
基因多态性
Interleukin-1 beta
Interleukin-1 receptor antagonist
Multiple sclerosis
Gene polymorphism
