摘要
利用ＳＤ大鼠肝细胞检测下上海市大气悬池颗粒物对ＤＮＡ损伤修复的影响。采用肝脏原位灌流分离肝细胞，用＾３Ｈ掺入地测定ＵＤＳ水平。结果表明：ＴＳＰ浓度为１－５０μｇ＝ｍｌ范围内，ＵＤＳ有较好的剂量－反应关系，当浓度达１００μｇ／ｍｌ时出现ＵＤＳ抑制。
This study detected the effect of TSP in Shanghai on the damage and reparement of DNA in SD rats' liver cells in vitro.The liver cells were collected with liver infusion in situ, and the level of UDS was detected with the method of ~3H incopretion.The result showed that a better dose-reponse relationship could be found in the range of 1μg/ml to 50μg/ ml TSP.The level of UDS was decreased when the dose of TSP is up to 100μg/ml.The level of UDS had significant difference between the negative control and the example, when the content of TSP in the example was up to 5μg/ml. We had also found the DNA damage potency of TSP in winter is stronger than that in spring.
出处
《环境与健康杂志》
CAS
CSSCI
CSCD
北大核心
1996年第3期105-107,共3页
Journal of Environment and Health
