摘要
10株胸膜肺炎放线杆菌参考菌株,经过纯化、扩增、提取基因组DNA,设计一套特异性引物,运用多重PCR技术,对外毒素基因apxⅠ、apxⅡ和apxⅢ进行扩增,产物经1.6%琼脂糖凝胶电泳,可将10株参考菌株分为5个血清群。血清Ⅰ群由259(血清1型)、263(血清5型)、267(血清9型)组成。血清Ⅱ群由260(血清2型)、262(血清4型)、264(血清6型)、266(血清8型)组成。血清Ⅲ群、血清Ⅳ群和血清Ⅴ群分别由261(血清3型)、265(血清7型)和268(血清10型)单独组成。对于血清Ⅰ群和血清Ⅱ群,通过apxⅣ进行扩增,结果259(血清1型)、263(血清5型)和267(血清9型)分别扩出2.4kb、2.8kb和1.6kb片段;262(血清4型)、264(血清6型)、260(血清2型)或266(血清8型)分别扩出1.6kb、2.0kb、2.8kb片段。
After purification, amplification and extracting genome DNA, 10 strains of reference Actinobacillus pleuropneumoniae were amplified using a set of specific primers designated for the apx Ⅰ , apx Ⅱ and apxⅢ genes by multiplex PCR. Their own products patterned by 1.6 % agarose gel electrophoresis showed 10 strain were divided into five serotype groups.Group Ⅰ included strain 259（serotype 1）, strain 263（serotypeS） and strain 267（serotypeg）. Group Ⅱ consisted of strain 260（serotype 2）, strain 262（serotype4） , strain 264（serotype6） and strain 266（serotype 8）. GroupⅢ, GroupⅣ and Group Ⅴ consisted of strain 261 （serotype 3）, strain 265 and strain 268 respectively. As for Group Ⅰ and Group Ⅱ, extoxin gene apxⅣ were amplified independently, 259（serotype 1）, strain 263（serotype5） and strain 267（serotype9） produce 2,4 kb, 2.8 kb and 1.6 kb special lanes; strain 262（serotype4） , strain 264（serotype6） , 260（serotype 2） or strain 266（serotype 8） produce 1.6 kb, 2.0 kb and 2.8 kb special bands.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第5期585-588,共4页
Chinese Journal of Preventive Veterinary Medicine
