摘要
采用AFLP技术,对湖南省宽皮柑橘类野生资源莽山野橘、道县野橘、江永野橘及印度野橘、立花橘等23个材料进行了DNA多态性鉴定.从64对引物组合中,筛选了8对引物组合,共得到334个扩增位点、4612条带数据,其中多态性位点294个,多态性位点比率88%.采用DICE相似系数UPGMA方法聚类,进行亲缘关系分析,23个材料多态性带比率为0.729～0.850,相似系数为0.68～0.91.根据聚类结果和亲缘关系分析,莽山尖叶和圆叶型野橘、道县粗皮小果型野橘、江永粗皮小果型野橘与印度野橘、立花橘亲缘关系较远,是完全不同的宽皮柑橘近缘野生种.道县野橘与江永野橘亲缘关系较近,应是同一种的不同地域类型.
AFLP technique was used to determine DNA polymorphism of the wild Mangshanyeju, Daoxianyeju, Jiangyongyeju, Citus India and Citrus tachibana and twenty-three cultivated germplasm resources of Citrus. Eight AFLP primer pairs was selected out of 64 and 334 PCR bands were obtained, among which 294 （88%） were polymorphic. The Dice＇ coefficient of the samples was 0.68~0.91. The results showed Mangshanyeju, Daoxianyeju, Mangshanyeju are different wild species of Eucitrus in comparison with Citrus India and Citrus tachibana. In addition to the existence and the wild disu-ibution of wild Citrus species in Nanling Mountains plus the DNA polymorphic data obtained in this study supported that Nanling Mountains is one of the native centers of the subgenus Eucitrus. The study also showed that there is a possible evolution tendency from the wild Mangshanyeju to cultivated species via Daoxian wild small fruit mandarin with rough rind, Daoxian wild larger fruit mandarin with smooth rind, Huapi mandarin among wild species of Eucitrus in Nanling mountains.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第4期385-388,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省科学技术厅项目(03NK3075)
关键词
宽皮柑橘
近缘野生种
亲缘关系鉴定
Citrus reticulata
wild relatives of Eucitrus
identification of relative relationships
