摘要
目的：观察牛带绦虫亚洲亚种感染乳猪后肝脏的病理学和组织化学改变。方法：以牛带绦虫亚洲亚种虫卵感染乳猪，封闭饲养；采用HE染色及组织化学染色观察不同时期肝组织的病理学变化并测定脂肪、糖原、蛋白质的含量，定量图像分析和SPSS统计学处理。结果：感染组感染4d时，肝脏开始有炎性细胞浸润；10—20d时，炎症反应加剧，Kupffer细胞增生，肝细胞水肿、部分气球样变，肝细胞有点片状坏死，中央静脉、肝血窦扩张和淤血；40-60d时，肝脏肉芽肿形成，肝脏呈早期肝硬化改变；70-80d时，部分肝脏呈肝硬化改变，胆管增生；感染不同时期均有不同程度脂肪含量的增多，糖原、蛋白质含量的减少，与正常对照组比较具有统计学意义（P〈0．01）；对照组乳猪无感染。结论：乳猪是牛带绦虫亚洲亚种的适宜中间宿主，牛带绦虫亚洲亚种感染可引起肝组织严重的病理学损害和组织化学改变。
Objective： To observe the pathological and histochemical changes in livers of porkets infected by Taenia saginata asiatica. Methods： Porkets were fed with eggs of T. saginata asiatica, and reared isolately in animal center to establish T. saginata asiatica infection model with normal porkets changes in porket livers after the infection were observed on liver sections stained with hematoxylin and eosin. Histochemical methods were adopted to check the content changes of lipid, glycogen and protein in the liver, and the data collected with image analysis system were analyzed statistically with SPSS. Results： T. saginata asiatica treated porkets were infected. Inflammatory reactions began on the 4th day, and progressed fast. Kupffer cell hyperplasia, hepatic hydropic degeneration and ballooning degenerations were found in 10 -20 days after infection. Hepatic central veins and sinusoids dilated and congested. Spotty necrosis ocurred in some local liver tissues; In 40 -60 days, granulomatous reactions and mild hepatocirrhosis were the main lesions. In 70 - 80 days, hepatocirrhosis and bile duct proliferation was observed in local liver. In different stages, lipid drops increased, glycogen and protein decreased to some degree. There were significant differences of metabolism between infected group and the control group （P 〈 0. 01 ）. Conclusions： Porket is favorable intermediate host of T. saginata asiatica, and its infection can result in serious pathological and histochemistry lesions in host organs.
出处
《贵阳医学院学报》
CAS
2006年第4期296-300,309,共6页
Journal of Guiyang Medical College
基金
国家自然科学基金资助项目(30260102)
