摘要
【目的】利用Xaf1诱导细胞株,检测Xaf1与一些细胞凋亡激动剂协同诱导肿瘤细胞凋亡的能力,初步探索Xaf1诱导凋亡的机制。【方法】Xaf1与细胞凋亡激动剂协同诱导的细胞凋亡由流式细胞检测DNA含量来表示,免疫荧光显微镜检测法与细胞核浆分离法检测Xaf1诱导细胞中Xaf1与XIAP的亚细胞分布,免疫沉淀法检测Xaf1与XIAP的关联性。【结果】流式细胞术DNA含量检测发现Xaf1与TNFα显著协同诱导细胞凋亡,凋亡峰值为49%,而Xaf1与阿霉素无协同作用,凋亡峰值为27%,免疫荧光显微镜检测及细胞核浆分离法结果显示Xaf1位于细胞核内,而XIAP却位于细胞质。【结论】Xaf1与TNFα显著协同诱导细胞凋亡,其协同机制可能不是解除XIAP对胱冬肽酶活性的抑制。
[ Objective ] Xaf1 inducible cell lines were used to detect the ability of Xaf1 cooperating with some apoptosis inducers and to investigate the mechanism of Xaf1 induced apoptosis. [ Methods ] The effect of Xaf1 cooperated with some apoptosis inducers on apoptosis was measured by flow cytometry. The sub-cellular localization of Xaf1 and XIAP in Xaf1 inducible cells was estimated respectively by immuno-fluoresces assay and cell fractionation assay. The association between Xaf1 and XIAP was detected by immuno-precipitation. [ Results ] Flow cytometry demonstrated that Xaf1 dramatically cooperated with TNFα to induce apoptosis, the apoptosis peak was 49%, but Xaf1 did not cooperate with adriamycin, the apoptosis peak was 27%. Immuno-fluoresces assay and cell fractionation assay showed that Xaf1 located in the nucleus while XIAP located in the cytosol. [ Conclusions ] Xaf1 cooperated with TNFα dramatically to induce apoptosis. The mechanism of cooperation may not be releasing XIAP mediated caspase inhibition.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2006年第4期374-377,382,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家中医药管理局基金资助项目(2005LHR08)
