摘要
目的:观察河豚毒素单用及河豚毒素与indoxacarb联合应用的镇痛抗炎作用。方法:实验于2004-09/2005-01在北京大学医学部神经精神药理实验室完成。选择ICR小鼠240只,按随机数字表法分为24组,每组10只。①醋酸扭体实验:110只小鼠分为阴性对照组、阳性对照组(吗啡,5mg/kg)、单用河豚毒素组(0.79,0.39,0.19μg/kg)、联合给药组犤河豚毒素(0.19μg/kg)+indoxacarb(1.4,2.8,28mg/kg),河豚毒素(0.19,0.39,0.79μg/kg)+indoxacarb(5.6mg/kg)犦。给药40min后,腹腔注射致痛剂6g/L冰醋酸溶液(0.01mL/g)。观察并记录15min内小鼠扭体反应次数。扭体抑制率(%)=(阴性对照组平均扭体次数-给药组平均扭体次数)/阴性对照组平均扭体次数×100%。②甲醛实验:50只小鼠分为阴性对照组、阳性对照组(吗啡,5mg/kg)、单用河豚毒素组(0.19μg/kg)、联合给药组犤河豚毒素(0.19μg/kg)+indoxacarb(1.4,2.8mg/kg)犦。给药40min后,小鼠右后足背皮下注射致痛剂9.2g/L甲醛(生理盐水配制)20μL/只。将小鼠舔、咬右爪的时间长度作为疼痛反应的指标。观察并记录0～5min,15～30min反应时间。抑制率(%)=(阴性对照组平均反应时间-给药组平均反应时间)/阴性对照组平均反应时间×100%。注射甲醛4h后,将小鼠断头处死,之后自膝关节切脚,称重,比较左右足差异,以此代表炎症程度。③坐骨神经慢性轻度结扎损伤实验:80只小鼠分为阴性对照组、阳性对照组(吗啡,5mg/kg)、单用河豚毒素组(0.19,0.39,0.79μg/kg)、联合给药组犤河豚毒素(0.19μg/kg)+indoxacarb(1.4,2.8,5.6mg/kg)犦。75mg/kg戊巴比妥钠腹腔注射麻醉小鼠。用细铜丝在坐骨神经上结扎2次。在术前及术后第1,3,5,7,9,11,13天测定热板潜伏期。第15天给药后15,30,45,60min测定小鼠热板潜伏期。结果:纳入动物240只,均进入结果分析。①单独使用河豚毒素(0.19,0.39,0.79μg/kg),扭体抑制率分别为22.2%,31.3%,39.2%,其抑制率与浓度存在剂量依�
AIM： To explore the analgesic and anti-inflammatiory effects of tetrodotoxin （TTX） alone versus TTX combined with indoxacarb. METHODS： The experiment was performed at the Laboratory of Neuropsychopharmacology, Peking University Health Science Center from September 2004 to January 2005. 240 ICR mice were selected and randomly assigned into 24 groups with 10 rats in each group. ① Writhing test： 110 mice were divided into negative control group, positive control group （morphine, 5 mg/kg）, TTX group （0.79,0.39 and 0.19 μg/kg）, TTX combined with indoxacarb group [TTX （0.19 μg/kg） ＋ indoxacarb （ 1.4,2.8 and 28 mg/kg） ,TTX （0.19,0.39 and 0.79 μg/kg）＋ indoxacarb（5.6 mg/kg）]. 40 minutes after drug administration, the animals were injected by intraperitoneal injection with 6 g/L glacial acetic acid （0.01 mL/g） and the writhing numbers within 15 minutes were recorded. Writhing inhibitive rate （%） was equal to （mean writhing number in the negative control group-mean writhing number in the administration group）/mean writhing number in the negative control group×100%. ②Formaldehyde test： Fifty mice were divided into negative control group, positive control group （morphine, 5 mg/kg）, TTX group （0.19 μg/kg）, TTX combined with indoxacarb group [TTX （0.19 μg/kg） ＋indoxacarb （ 1.4,2.8 mg/kg）]. Forty minutes later, formaldehyde （9.2 g/L in saline, 20 μL/mouse） was injected subcutaneously into the dorsum of the mouse right hindpaw. Duration of licking and biting right claw was used as pain reaction index, The time the animals spent licking the injected hindpaw was observed and recorded between 0 and 5 minutes and 15 and 30 minutes. Inhibitive rate （%） was equal to （mean reaction time in the negative control group-mean reaction time in the administration group）/mean reaction time in the negative control group×100%. The mice were killed by decapitation 4 hours after formaldehyde injection, and both hindpaws were amputated at the knee joint and
出处
《中国临床康复》
CSCD
北大核心
2006年第34期115-118,共4页
Chinese Journal of Clinical Rehabilitation
