摘要
新疆地区盐湖密布,蕴藏着丰富的微生物资源。为保护和利用微生物物种与基因资源,作者从新疆准噶尔盆地的艾比湖和天山山间盆地的伊吾湖分离纯化嗜盐微生物。采用PCR方法扩增其中65株嗜盐古菌16SrRNA基因序列。序列分析表明,分离的嗜盐古菌分属6个属,艾比湖以Haloterrigena和Natrinema属的菌株为主,伊吾湖由Haloarcula和Halorubrum两个属的菌株构成。通过多样性指数、丰富度指数、均匀度指数和物种相对多度模型对分离的菌株进行多样性分析和比较,结果表明,盐湖嗜盐古菌的多样性指数、丰富度指数和均匀度指数具有一定相关性,艾比湖可培养嗜盐古菌的多样性高于伊吾湖。研究发现了一些新的物种资源,表明新疆盐湖中孕育的特色微生物资源亟待保护与利用。
The diversity of halophilic microorganisms from two different hypersaline environments was analyzed and compared by the polymerase chain reaction （PCR）-based molecular technique and cultivation approaches. The samples were collected from Aibi Lake located in Zhungeer Basin and Yiwu Lake in Tianshan Basin, Xinjiang, respectively. Under aerobic culture conditions, novel halophiles were isolated. 16S rRNA gene sequences of 65 isolates were amplified and determined. The results revealed that all sequences belonged to six genera grouped within the Halobacteriaceae. Most of the 16S rRNA gene sequences related to the genera Haloterrigena and Natrinema were detected in Aibi Lake samples. In contrast, sequences related to the genera Haloarcula and Halorubrum were obtained from Yiwu Lake samples. In addition, the isolates＇ diversities were analyzed and compared using different diversity indices, richness indices, evenness indices, and species abundance models. There were certain correlations among these indices, and they indicated that halophilic archaea diversity of Aibi Lake is higher than that of Yiwu Lake. The discovery of novel species, in a relatively small number of sites from two representative salt lakes, indicated that there are many microbial resources in Xinjiang region that should be protected and utilized.
出处
《生物多样性》
CAS
CSCD
北大核心
2006年第4期359-362,共4页
Biodiversity Science
基金
国家重点基础研究发展规划项目(2004CB719604-3)
中国博士后科学基金(2005038636)
