摘要
目的探讨Fhit、Caspase-8基因在膀胱肿瘤组织中的表达及其意义。方法应用免疫组化Elivision法检测Fhit、Caspase-8基因在正常膀胱组织、腺性膀胱炎、膀胱癌旁组织、膀胱癌组织中的表达;用图像分析系统测量Fhit、Caspase-8的平均光密度值。结果Fhit、Caspase-8在正常膀胱组织、腺性膀胱炎、膀胱癌旁组织、膀胱移行细胞癌、膀胱腺癌中的平均光密度值分别为:0.270±0.042、0.248±0.044;0.142±0.040、0.150±0.051;0.240±0.061、0.234±0.053;0.097±0.053、0.108±0.053;0.089±0.026、0.105±0.062。膀胱癌中Fhit、Caspase-8的表达明显低于正常膀胱组(P<0.01),Fhit、Caspase-8在膀胱移行细胞癌中的表达随病理分级上升而减弱(P<0.01),且与分级呈负相关(r分别为-0.700、-0.674,P均<0.01);但与病理分期无关(r=0.456,P=0.025;r=0.337,P=0.107)。Fhit、Caspase-8在膀胱组织中的表达呈正相关(r=0.726,P<0.01)。结论膀胱癌中Fhit表达减弱或缺失伴随着Caspase-8表达的下调,提示膀胱癌的演进过程与Fhit、Caspase-8有密切关系。
Objective To study the role of Fhit.Caspase-8 in the development of carcinoma of bladder, as well as its clinical significance. Methods The expressions of Fhit and Caspase-8 were measured by immunohistochemistry in the normal bladder samples, cystitis glandularis, paraneoplastic samples and carcinoma of bladder, and their average optical was detected by image analysis system. Results The average optical of Fhit and Caspase-8 in normal bladder samples.cystitis glandularis.paraneoplastic samples.transitional cell carcinoma of bladder and adenocarcinoma of bladder were 0. 270±0、042、0. 248±0、044;0. 142±0. 040、0. 150±0、051 ;0. 240±0. 061、0. 234±0. 053;0. 097±0. 053、0. 108±0. 053;0. 089±0. 026、0. 105±0. 062 respectively. The expression of Fhit and Caspase-8 in carcinoma of bladder was significantly lower than that in normal bladder samples （P〈0.01）. The intensity of Fhit and Caspase-8 expression was decreased with the increasing of grades of tumor （P〈0.01）, and obviously correlated with the grades respectively （r =-0. 700,r=-0. 674,P〈0、01）, but incorrelated with stages of tumor（r=0. 456, P=0. 025 ;r = 0. 337, P= 0. 107）. A positive correlation was found between Fhit protein loss and Caspase-8 reduced expression（r=0. 726 ,P〈0. 01）. Conclusion The down regulation of Caspase-8 in bladder neoplasm in connection with Fhit protein loss reeeals the close relationship between Fhit . Caspase-8 and the occurrence and progression of the cancer.
出处
《江西医学院学报》
CAS
2006年第4期107-110,共4页
Acta Academiae Medicinae Jiangxi
