摘要
目的探讨系统发育在SARS病毒分类中的应用。方法系统发育分析是通过构建系统树来评价物种或分子之间进化关系的一种方法。本文采用目前比较成熟、应用较为广泛的系统发育分析软件PHYLIP,根据GenBank中14个冠状病毒、17个SARS病毒和来自Flaviviridae和Togaviridae两个科的4个单链RNA病毒的完全基因组数据,选用UPGMA距离法构建系统树,构建SARS病毒分类。结果SARS病毒在冠状病毒中自身构成一个独立分支,与哺乳动物为宿主的冠状病毒的亲缘关系最近。结论系统发育树可以用于新型病毒的分类。
Objective Discussed the classification of SARS viruses. Methods This paper introduced the theory and methods of constructing phylogenetic trees, and demonstrated the PHYLIP software, which is widely used in the field. Based on the whole - genome from 14 coronary viruses, 17 SARS viruses and 4 single - strained RNA viruses in Flaviviridae and Togaviridae family, the SARS viruses was classified by means of constructing phylogenetic tree with UPGMA method on the platform of PHYLIP 3.6. Results The SARS viruses should be classified as a distinct branch from other coronary viruses, and have the nearest kindredship to the coronary virus which guest in mammal. Conclusion Phylogenetic analysis is a tree method to evaluate the phylogenetic relationship of molecular sequences or species, and can be used in the classification of new viruses.
出处
《中国卫生统计》
CSCD
北大核心
2006年第4期315-318,共4页
Chinese Journal of Health Statistics
