摘要
目的本实验观察挤压家兔后肢导致肝氧化损伤,探讨氧化应激在挤压家兔后肢导致肝损伤中的作用。方法用标准重物间断压迫家兔后肢复制模型。采用生化方法检测ALT和AST活性;分析天平称重方法检测肝脏湿干重比值(W/D);比色法测定肝组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性和总抗氧化能力(T-AOC)以及丙二醛(MDA)含量变化;并观察肝组织病理学改变。结果血清ALT和AST活性明显增高(P<0.01),肝细胞明显肿胀,肝窦轻度淤血,而肝脏湿干重比值(W/D)轻度增加(P<0.05);肝脏氧化损伤明显加重,MDA含量增高,而肝脏抗氧化能力下降,SOD、CAT、GSH-Px活性和T-AOC分别比对照组降低了17%、29%、24%和21%(P均<0.01)。结论挤压家兔后肢引起急性肝损伤及肝脏的抗氧化能力下降;肝脏局部抗氧化能力下降可能是肝损伤的重要因素。
Objective To investigate the role of oxidative stress in acute liver injury during crushing hindlimbs in rabbit. Methods The crushing injury model in rabbit was established by intermittent crushing the hind limbs of rabbit with standard weight. The ALT and AST activities were spectrophotometrically measured. The weight ratio （wet/dry,W/D） of livers was measured with scale, and the pathologic changes were observed. Superoxide dismutase （SOD）, eatalase （CAT）, glutathione peroxidase （GSH-Px） and total anti-oxidant capacity （T-AOC） as well as malondialdehyde （MDA） level were spectrophotometrically measured. Results As compared with control rabbits, crushing hindlimbs of rabbits induced acute liver injury with the increase in ALT and AST activities in serum,which were 4.3l （P〈O.O1） and 10.54 times （P〈0.01） of control group respectively, there were cellular swellings and slight congestion of hepatic sinuses. In addition,crushing hind-limbs elicited significant decrease in SOD,CAT, GSH-Px activity and TAOC to 17%,29%,24% and 21% （P〈0.01）compared with control group respectively, whereas MDA level markedly enhanced. Conclusion Crushing hindlimbs of rabbits induced acute liver injury and significant decrease in anti-oxidant capacity, the latter maybe play an important role in crushing hind-limbs of rabbits-elicited the acute liver injury.
出处
《法医学杂志》
CAS
CSCD
2006年第4期248-250,共3页
Journal of Forensic Medicine
关键词
肝脏
氧化应激
抗氧化能力
挤压伤
liver
oxidative stress
antioxidant capacity
crushing injury
