摘要
目的:研究细胞因子体外联合诱导培养的慢性粒细胞白血病(CML)患者树突状细胞(DCs)的表型和抗原递呈、分泌和迁移功能的变化。方法:分离CML患者(CML组)和健康志愿者(正常组)外周血单个核细胞(PMNCs),经培养获取贴壁细胞,将CML组和正常组的贴壁细胞及K562细胞用细胞因子rhGM-CSF、rhIL-4、TNF-α体外诱导10d后,流式细胞仪检测细胞的免疫表型;培养5d后,用ELISA检测细胞的内吞功能;应用混合淋巴细胞培养分析3组DCs对静息T细胞的免疫刺激活性。结果:正常组、CML组、K562组DCs的特异性标志CD83和共刺激分子标记CD80和CD86以及CD11和HLA-DR的表达差异无统计学意义(P>0.05);DCs的抗原吞噬、迁移能力和对T细胞的免疫刺激活性,CML组和K562组之间差异无统计学意义(P>0.05),CML组和正常组间差异有统计学意义(P<0.05)。结论:CML患者DCs的免疫表型和正常人相似,但其抗原捕获、递呈和迁移功能有缺陷。
Aim： To study the antigen processing and presentation, secretion, and migration ability of dendritic cells （DCs） from chronic myeloid leukemia （CML） patients. Methods：The differentiation of DCs generated from CD34^＋ progenitor cells was mediated by cytokines like granulocyte macrophage colony-stimulating factor, tumor necrosis factor, interleukin-4. Maturation status of CML DCs and normal DCs were evaluated by the surface marker using flow cytometry. Ability of DCs to stimulate T cells and capture antigen were detected using a liquid-scintillation counter and ELISA. Results： Phenotypic analysis of CML and normal DCs showed a similar surface expression of maturation makers of CD80, CD86, CD83, CDll, and HLA-DR （P 〉 0.05）. There are statistical differences in the capacity of T cells activation, endocytosis or phagocytosis and transmigration between DCs derived from CML patients and healthy individuals. Conclusion： DCs derived from both healthy subjects and patients with CML differentiated and matured cultured in vitro in a similar way. However, DCs generated from C ML patients have a reduced capacity to capture,process and present antigen and migration when compared with DCs from healthy controls.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2006年第5期895-897,共3页
Journal of Zhengzhou University(Medical Sciences)
关键词
慢性粒细胞白血病
树突状细胞
抗原递呈
迁移
chronic myeloid leukemia
dendritic cell
antigen presentation
migration
