摘要
微生物降解是环境中芳烃类污染物处理的重要手段与方法,研究降解菌暴露在污染物下蛋白差异表达的情况,可以揭示微生物利用有机物做为碳源与能源的途径与方法.本项目以蛋白质二维电泳研究结果为基础,对产碱假单胞菌在污染物胁迫下表达的蛋白质点进行N端测序分析,对诱导性的同工酶进行了克隆与测序分析.结果显示,产碱假单胞菌在污染物胁迫下能表达与细菌的趋化性密切相关的蛋白,在研究的菌株中存在同工不同源的酶系统.
Microbiological deterioration is an important method in the treatment of aromatic hydrocarbon. The research of differential expression of degradation strain stressed by pollutants will reveal the pathway by which microorganism utilize organics as carbon and energy source. The N - terminal of several protein spots selected from the 2D-Gel result were sequenced when Pseudomonas alcaligenes SNZ28 was stressed with pollutants. Partial GDO II PCR product was amplified and cloned. The results showed that the bacterium stressed with pollutant would express chemiotaxis protein and an isoenzyme system was existed in aromatic hydrocarbon pathway with low homologous in this strain.
出处
《上海师范大学学报（自然科学版）》
2006年第4期65-69,共5页
Journal of Shanghai Normal University(Natural Sciences)
基金
国家自然科学基金项目(20337010)
国家基础研究重大项目(2004CB418503)
上海市基础研究重大项目(04JC1405)
上海生态学重点学科建设资助
关键词
芳香烃
生物降解
蛋白质组学
Aromatic hydrocarbon
biodegradation
proteomic
