摘要
目的初步探讨人脑星形细胞肿瘤中新生微血管的管壁组成成分与微血管构筑异质性(tumor microvascular architecturphenotype heterogeneity,T-MAPH)之间的关系。方法收集45例人脑星形细胞肿瘤标本,选择其新生微血管形态不同的“热点区域”制备组织芯片;在此基础上采用免疫组织化学染色技术分别进行GFAP、CD34和α-SMA标记,观察微血管管壁组成细胞的免疫表型与其不同形态特征之间的关系。结果成功构建了含幼稚微血管、厚壁微血管、肾小球样微血管及薄壁、蛇行状微血管等各种形态特征的153点组织芯片;各种形态的微血管中均可检测到呈单层排列、位于微血管最内层的CD34阳性的内皮细胞;α-SMA阳性反应可见于内皮细胞外相当于周细胞处,其阳性表达范围及数目在不同形态的微血管中呈现多样性:幼稚微血管仅见少量表达,而在厚壁和“肾小球样”微血管中α-SMA阳性细胞多呈活跃增生的单层或多层。结论星形细胞瘤微血管形态构筑的多样性(异质性)是由内皮细胞和α-SMA阳性周细胞共同参与形成的,而后者的作用可能更为突出和重要。
Purpose To investigate the relationship between the tumor microvascular architecture phenotype heterogeneity （T-MAPH） and the mural components of the microvessels in human brain astrocytic tumor. Methods The various microvessel “hot spot” areas based on the morphological features were selected from 45 cases of human astrocytic tumor specimens, and were used to construct tissue microarrays. The expression and localization of glial fibrillary acidic protein （GFAP） , CD34 and α-smooth muscle actin （α-SMA） were detected on the tissue microarrays with immunohistochemical staining. Results The tissue microarrays of 153 tissue cores containing glioma microvessels with various morphological features, such as infantility microvessels, thick-and thin-wall microvessels, and glomeruloid microvessels etc, and normal brain tissues were successfully constructed. Immunohistochemically, the endothelial cells in almost every newly formed microvessels were positive for CD34, which was located in inner layer of the microvessels, α-SMA positive reaction was localized at pericytes surrounding the endothelial cells. The amounts and extensions of α-SMA positive-cells presented polymorphism among different microvessels. It is only few α-SMA positive staining in infantility microvessels, whereas monolayer or multilayer positive-cells were detected in thick-wall or glomeruloid microvessels. Conclusion The endothelial cells and α-SMA-positive pericytes might mainly contribute to the polymorphism of new-formed microvessels in astrocytic tumor, and α-SMA-positive cells contributed particularly to T-MAPH in astrocytic tumor.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2006年第4期440-443,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(30270526
30370552)
第三军医大学西南医院临床研究专项基金(SWH20056009)
关键词
脑肿瘤
星形细胞瘤
血管生成
周细胞
肿瘤微血管构筑表型
组织芯片
brain neoplasms
astrocytoma
angiogenesis
pericyte
tumor microvascular architecture phenotype （T-MAP）
tissue microarray
