摘要
目的探讨γ射线和微波复合照射对造血系统的损伤特点及规律,为进一步研究其损伤机制提供理论和实验基础。方法216只雄性昆明小鼠随机分为对照组、γ射线照射组(5·5Gy,简称射线组)、微波照射组(50mW/cm2,简称微波组)及γ射线与微波复合照射组(5·5Gy+50mW/cm2,简称复合组)。60Coγ射线(剂量率为91·1cGy/min)全身一次性照射后立即进行微波全身辐照(S波段,平均功率密度为50mW/cm2)5min。分别于照射后6h及1、3、7、14、28、90、180天活杀取材,观察胸骨骨髓组织学变化,并采股静脉血行常规检测,同时对复合照射后6h及第1、3、7天的胸骨骨髓进行超微结构观察。结果组织学观察发现无论γ射线还是复合照射均可导致骨髓组织经历典型的凋亡坏死、空虚、再生修复及恢复4个阶段的病理改变,但复合组的病变重于射线组,恢复也更为迟缓。外周血象检查发现小鼠经γ射线及复合照射后白细胞、红细胞、血小板数和血红蛋白含量均呈进行性下降,于照射后期逐渐恢复,但复合组比射线组下降更明显,恢复也更缓慢。超微结构观察发现复合照射后骨髓造血细胞出现明显的变性、凋亡及坏死等改变,以照射后6h最明显。结论一定剂量的γ射线和一定强度的微波复合照射可严重损伤小鼠造血系统,表现为骨髓组织发生明显的形态学改变和功能障碍,其损伤效应主要以γ射线为主,微波在一定程度上加重了γ射线的损伤。
Objective To explore the patterns and features of biological effects of γ-ray irradiation combined with microwave on the mouse hematopoietic system, and to provide theoretical and experimental basis for understanding the possible mechanism of the bone marrow injury caused by the combined action of microwave and γ-ray irradiation. Methods 216 healthy KM mice were randomly divided into the following four groups： normal control, microwave （S frequency range, 50mW/cm^2）, γ-ray irradiation （5. 5Gy）, microwave combined γ-ray radiation （5. 5Gy ＋ 50mW/cm^2 ）. They were sacrificed at 6h, 1, 3, 7, 14, 28, 90 and 180 days after radiation, respectively, then the histological and ultrastructural changes in the bone marrow and the peripheral hemogram were observed. Results Histopatbological changes： the bone marrow appeared to be obviously injured either by radiation or microwave exposure, characterized by undergoing four phases, namely apoptosis-necrosis, void, regeneration and recovery phase. However, the pathological changes were more obvious and the recovery was slower in microwave combined y-ray radiation group. Peripheral hernogram： the numbers of leucocytes, erythrocytes and platelets, and the content of hemoglobin decreased in both γ-ray irradiation group and microwave combined γ-ray radiation group, and the decrease in microwave combined radiation group was more remarkable. Ultrastructure： the bone marrow hematopoietic cells underwent obvious degeneration, apoptosis and necrosis in microwave combined γ-ray radiation group especially at 6 hours after radiation. Conclusion γ-ray combined with microwave could induce hematopoietic dysfunction and pathomorpbological changes in hematopoietic organ, which were mainly caused by γ-ray, and the changes were aggravated.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2006年第8期790-793,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金重点课题(30230360)
关键词
Γ射线
微波
骨髓
造血细胞
gamma rays
microwaves
bone marrow
hematopoietic cells
