摘要
通过计算机分析国际分子生物学数据库和ｃＤＮＡ序列分析等方法，在水稻（Ｏｒｙｚａｓａｔｉｖａ）中发现了一种新的核仁小分子ＲＮＡ：Ｃ１ＳｎｏＲＮＡ．该ＲＮＡ具有ｂｏｘＣ，ｂｏｘＤ和末端碱基配对区等典型的核仁小分子ＲＮＡ结构特征；并有１４个核苷酸与水稻１８ｓｒＲＮＡ中一段保守序列互补．推测Ｃ１ＳｎｏＲＮＡ可能在１８ｓｒＲＮＡ甲基化过程中起重要作用．编码Ｃ１ＳｎｏＲＮＡ的基因位于水稻Ｈｓｐ７０基因的第一个内含子中．这是在水稻中发现的第一个由基因内含子编码的ＳｎｏＲＮＡ．
With computer analysis on Genbank database and cDNA sequencing methods, a novel intron-encoded SnoRNA, termed C1 SnoRNA, from rice(Oryza sativa) was identified. The RNA possesse the structural feature (boxC, boxD and a terminal stem)typic of SnoRNA, as well as with a 12 nt complementary to a conserved sequence of rice 18s rRNA, which implies a possible function of C1 SnoRNA in 18s rRNA methylation. The gene coding for C1 SnoRNA is nested in the first intron of rice Hsp70 gene. This is the first report on intron-encoded SnoRNA characterized from rice and plant.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
1996年第3期1-5,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家杰出青年基金
