摘要
用沙门氏菌O,H,Vi抗原免疫BALB/c小鼠,采用细胞融合技术,获得分泌抗沙门氏茵0-2,4,7,8,9,3,10,11和H-a,b,c,d,i及Vi抗原的单克隆抗体杂交瘤细胞系29株。所产生的抗体17株属IgM,7株为IgG3,3株为IgG1,1株为IgG_(2a),1株未测;轻链除3株为λ链外,其余为κ链。将杂交瘤细胞所诱生的腹水,组成一套(11种)沙门氏菌诊断用单克隆抗体试剂,经用沙门氏菌属和其它肠道菌属的代表菌株作鉴定,证明具有良好的特异性和敏感性、可供初步诊断伤寒,副伤寒和鼠伤寒用。与目前常规生产的家兔诊断血清相比,具有简化生产过程,节约家兔与培养基及省时省力等优点,值得在生产上和使用上推广。
BALB/c mice were immunized with Salmonella strains containing different. Hf and Vi antigens or purified If and V i antigens. The spleen cells were fused with myeloma cells Sp2/0. 29 strains o f hybridoima cell secret ing monolonal antibodies againsl Salmonella antigen 0:2,4, 7, 8, 9, 3, 10, 11, H:a, b, c, d, i and Vi respectively, were obtained by screening. Among the 29 strains, 17 belonged to IgM, 7 lgG3, 3 IgC1, 1 IgG2a, but 1 was not determined. 3 of them were λ chain and the rest,k chain. McAb asciles were determined with represcnlative strains of Salmonella and other enlerobaeteria. Most of them possessed high specificity and sensitivity. A kit of dia-gnoslic reagents of Salmonella MeAbs(11 kinds)was produced for the use of preliminary diagnosis of S. typhi, S. paratyphi and S. typhimurium. The production processes of I he Salmonella McAbs were more convenient than that of the traditional products. Thus it would benefit in the use of them.
关键词
沙门氏菌
杂交瘤
单克隆抗体
antibodies, monoclonal
hybridoma
Salmonella
reagent kits, diagnostic.
