摘要
应用淋巴细胞杂交瘤技术,制备了与沙门氏菌属诊断血清相对应的成套11种单克隆抗体诊断试剂,包括:抗O-2,4,7,9;抗H-a,b,c,d,i;抗Vi和抗A-F群“O”多价抗体。经711株已知菌种(包括沙门氏菌563株,非沙门氏茵148株)采用玻片凝集试验进行考核鉴定,结果证明:它们只选择性地与具有相应抗原的菌株出现快速而明显的凝集,而不与其它菌株发生交叉反应,其特异性和敏感性达到并超过目前的沙门氏茵诊断血清制检规程的要求。表明这组单克隆抗体完全可以取代以往用传统方法生产的家兔免疫诊断血清,用于沙门氏菌的初步鉴定。
A kit of Salmonella monoclonal antibodies against 11 kinds of anligenic factors of Salmonella strains commonly occurred in nature was established by hybrid oma technique. It included anli 0-2, 1, 7, 9, anti H-a, b, c, d, i, anti Vi and anti A-F 'O' group McAbs. It was tested by 711 known strains ( 563 Salmomella strains, 148 other strains) by using slide agglutination test and showed that the McAbs only agglutinated rapidly and clearly with the strains which possessed the relative antigens and did not agglutinate with non-relating enterobacterial strains. The specificity and sensitivity of these Salmonella McAbs were reached and even exceeded the approved the approved requirements in effect when tested with appropriate strains and methods. It is resonabbly concluded that these McA.bs can be used as cffctive diagnosis reagents instead of the original ones.
关键词
沙门氏菌
试挤箱
单克隆抗体
Salmonella
antibodies, monoclonal
reagent kits, diagnosis.
