摘要
将外源性凝集素刀豆素A包被于ELISA板上,与检测血清或胃液反应后,封闭,用单抗MGd1-酶标SPA检测,以探讨鼠抗人胃癌单抗MGd1相应抗原(MGd1-Ag)在胃癌诊断中的意义。共检测血清183份(胃癌患者142例,正常人41例),胃液97份(胃癌患者72例,良性胃病患者25例)。结果显示:在118例未手术胃癌患者中,阳性58例,占49.2％;而24例胃癌切除术后患者中阳性4例,占16.7％;正常人阳性者为24.0％。胃癌患者胃液阳性率为41.7％,而良性胃病患者仅为4.0％。实验结果提示血清、胃液之中MGd1-Ag的检测对胃癌具有一定的诊断价值。
ELISA plates were coated with the concanavalin A at 4℃ for 12 hours, and then to cach of them the sera and gastric juice were added and incubaied for 4 hours. The MGd1-Ag in sera and gastric juice was determined by MGd1. It was found that the positive rates were 49.2% ( 58/118 ) in sera of patients with gastric cancer; 16.7% in sera of patients with gastric cancer, who underwent gastrcctomy and 2.4% in sera of normal persons. In gastic juice of patients with gastric cancer, the positive rates were 41.7%' and benign diseases were 1.0%. It suggests that determination of MGdl-Ag can be used in diagnosis of gastric cancer.
关键词
胃肿瘤
血清诊断
单克隆抗体
stomach neoplasms
anlibodies, monoclonal
serodiugnosis
