摘要
目的观察二次打击致脓毒症大鼠血清血小板生成素(TPO)水平的改变,并初步探讨脓毒症时血清 TPO 水平与脓毒症严重程度的关系。方法将 SD 大鼠分成健康对照组(A,n=10)、手术对照组(B,n=8)、脓毒症组(C,n=15)、严重脓毒症组(D,n=12)和脓毒性休克组(E,n=8)。通过低血容量休克打击后注射不同剂量(1mg/kg、2mg/kg、3mg/kg)大肠杆菌内毒素(LPS)复制脓毒症、严重脓毒症及脓毒性休克模型。24h 后测定大鼠血小板计数和血清 TPO、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果脓毒症组血小板较健康对照组无明显变化,严重脓毒症组和脓毒性休克组血小板计数下降显著(P<0.01)。TPO 水平在脓毒症组开始升高,在严重脓毒症组和脓毒性休克组显著升高水平(P<0.01)。IL-6、TNF-α在脓毒症组、严重脓毒症组和脓毒性休克组逐渐升高。多元线性回归分析显示:TPO 与 IL-6密切相关(r=0.832,P<0.00)。结论脓毒症大鼠血清 TPO 水平升高,脓毒症严重程度是 TPO 水平主要决定因素,TPO 水平可作为脓毒症严重程度的生物学标志。
Objective To measure serum thrombopoietin levels in two hits of induced septic rats and to investigate their relationship with sepsis severity. Methods SD rats were randomly divided into five group - control group（ A, n = 10）, injury group（ B, n = 8）, sepsis group（ C, n = 15 ）, severe sepsis group（ D, n = 12）, septic shock group（ E, n = 8 ）. A rat model of sepsis was established by infusing lipopolysaccharide（LPS） at a dose of 1 mg/kg,2 mg/kg ,3 mg/kg after hemorrhagic shock by bleeding. After blood sampling, serum thrombopoietin, platelet count, intedeukin- 6（ IL- 6） and tumor necrosis factor-α（TNF-α） were measure. Results Platelet counts did not decease in sepsis group but they significantly decreased in severe sepsis group and septic shock group（ P 〈 0.01 ）. In contrast thrombopoietin levels increased in sepsis group , exhibiting further significant increase in severe sepsis group and septic shock group. IL-6 and TNF-α increased in injury group, sepsis group, severe sepsis group and septic shock group. In multiple regression analysis, thrombopoietin was significantly related to IL- 6. Conclusion Thrombopoietin levels increased in septic rats. Sepsis severity was the major determinant of elevated thrombopoietin levels, In septic rats. Thrombopoietin represented a potential marker of sepsis severity.
出处
《临床急诊杂志》
CAS
2006年第4期157-159,共3页
Journal of Clinical Emergency
