摘要
目的分析127份黑大豆种质的总抗氧化能力及与其总酚和花色苷含量之间的差异和相关性。方法各类型之间差异采用平均数的t测验,聚类分析采用快速聚类法,相关性分析采用相关系数法。结果127份黑大豆种质种皮的总抗氧化能力、总酚和花色苷含量的变幅分别为0.44～3.56mmol.g-1、7.05～74.82mg.g-1和0.22～1.87mg.g-1,表现出明显的基因型差异,不同类型及不同生态区黑大豆种质的总抗氧化能力、总酚和花色苷含量差异较大,其中,黑大豆/黄大豆、黑大豆春播/秋播、夏播/秋播、东北春/南方春、东北春/南方夏、北方春/南方春、北方春/南方夏之间的差异分别达极显著(P<0.01)或显著(P<0.05)水平,总体趋势是北方春最高,东北春次之,南方春最弱。127份黑大豆种质可聚成6大类群,分别由3、24、20、31、37和12份种质组成。黑大豆的总抗氧化能力与其中的总酚和花色苷含量之间呈现极显著(P<0.01)正相关性。结论黑大豆的抗氧化作用与其种皮中所含的多酚和花色苷类物质关系密切。
[Objective] To study the correlations between antioxidations, contents of total phenolics and anthocyanin in 127 accessions of black soybean. [Method] A t-test, a fast clustering procedure and a correlation coefficient analysis were used for experimentation. [Result] The ranges of total antioxidant capacity （TAC）, total phenolics and anthocyanin contents in 127 black soybean accessions were 0.44-3.56 mmol·g^-1, 7.05-74.82mg·g^-1 and 0.22-1.87mg·g^-1, respectively, displaying significant genotype differences. The big differences in TAC, total phenolics and anthocyanin contents existed among various types of accessions from geographical regions. The differences between accessions from black and yellow soybeans, Spring and Autumn, Summer and Autumn, Dongbeichun and Nanfangchun, Dongbeichun and Nanfangxia, Beifangchun and Nanfangchun, Beifangchun and Nanfangxia were significant at 0.01 or 0.05 levels respectively. The general tendency was that the TAC, total phenolics and anthocyanin contents of Beifangchun accessions were higher than those of Dongbeichun ones, those of Nanfangchun accessions were the least. 127 black soybean accessions could be clustered into 6 clusters which consist of 3, 24, 20, 31, 37 and 12 accessions, respectively. Most significant （P〈0.01） correlations existed respectively between the TAC and total phenolics content, TAC and anthocyanin content of black soybean. [Conclusion] The results showed total phenolics and anthocyanin in black soybean seed coat were the important antioxidation substances.
出处
《中国农业科学》
CAS
CSCD
北大核心
2006年第8期1545-1552,共8页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(30200171)
关键词
黑大豆种皮
抗氧化作用
总酚含量
花色苷含量
相关性
Seed coat of black soybean
Antioxidation
Total phenolics content
Anthocyanin content
Correlation
