摘要
以￣３Ｈ－甲基掺入法测定大鼠脑中ＤＮＡ甲基化酶活性，发现在长期服用神方补肾生血药后，大鼠脑ＤＮＡ甲基化酶比活力增高，酶的热稳定性和对ＮａＣｌ的耐受力略有增强，最适ｐＨ改变。用ＳＤＳ－ＰＡＧＥ检测酶粗提物的蛋白成分，发现给药组电泳行为有所改变。说明神方补肾生血药改变大鼠脑ＤＮＡ甲基化酶活性和酶学性质，从而影响其ＤＮＡ甲基化水平，可能是该药物抗衰老作用的途径之一。
DNA methylase activity of brain in Wistar rats treated with Shenfang Bushen Shengxue Drug(SBSD) was observed using 3H-labelled methyl group of S-adenosyl-methionine incorporated into DNA.It was found that the SBSD has a marked effect on anti-aging. After SBSD treatment the specific activity of DNA methylase increased, the thermostability and salt-tolerance of it improved slightly,its optional PH changed from 7.5 to 8.0.A and a difference was found between the electrophoresgram of partially purified product of DNA methylase in SBSD treated rats and that in normal rats,suggesting SBSD could change the activity and characteristics of DNA methylase so as to affect the level of DNA methylation,which might be one pathway of SBSD effects on anti-aging.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
1996年第6期358-359,共2页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金
