摘要
目的:建立烧烫伤凝胶(黄连,黄柏,大黄等)中5种蒽醌成分的含量测定方法。方法:采用HPLC法,伊利特-C18柱,以甲醇-1%磷酸溶液(80∶20),流速:1.0 mL/m in,检测波长:430 nm。结果:该方法使样品中5种蒽醌类成分得到良好的分离,且线性关系良好。大黄素、大黄酚、大黄酸、大黄素甲醚、芦荟大黄素的平均回收率及RSD分别为98.0%,1.3%;97.1%,1.8%;97.8%,1.5%;97.4%,1.7%;96.7%,1.2%。结论:本法专属性强,灵敏度高,重现性好,适于该制剂中蒽醌类成分的分离与测定。
AIM： To establish the method of determining five anthraquinone components in Shaotangshang Gel （Rhizoma Coptidis, Cortex Phellodendri Chinensis, Radix et Rhizoma Rhei, etc. ）. METHODS：HPLC was used to determine emodin, chrysophanol, rhein, physcion, alone-emodin in Shaotangshang Gel. The separation was performed on Yilited- C18 Column with MeOH-phosphate acid （0.1% ）（ 80： 20） as the modile phase, the flow rate was 1.0 mL/min and detection wavelength was set at 430 nm. RESULTS ： The resolution and the linearity of the method were good with the average recoveries and RSD values of emoldin, chrysophanol, rhein, physcion, aloe-emodin of 98.0%, 1.3% ;97.1%, 1.8% ;97.8%, 1.5% ;97.4%, 1.7% ;96.7%, 1.2% respectively. CONCLUSION ： This method is sensitive, accurate and can be used for the quality control of Shaotangshang Gel.
出处
《中成药》
CAS
CSCD
北大核心
2006年第8期1120-1122,共3页
Chinese Traditional Patent Medicine
