摘要
目的探讨二甲胂酸（DMAA）诱导胃癌细胞SGC-7901的凋亡作用。方法用不同浓度的二甲胂酸与人的胃癌细胞SGC-7901共育一段时间后观察细胞的存活、形态学改变及生物学变化。结果其中以0．5～5．0mmol／L浓度的DMAA诱导其凋亡的作用最明显。主要形态学表现为细胞膜完整、染色质固缩、核碎裂、凋亡小体形成；电泳结果上则显示明显的梯形凋亡条带。而当DMAA浓度≥10mmol／L时细胞核和细胞质中的荧光减弱，细胞呈坏死趋势；同时，MTT结果显示随着DMAA浓度的升高，细胞的活性明显降低。结论DMAA在低浓度时能有效诱导胃癌细胞SGC-7901凋亡，而高浓度时主要导致其坏死。
Objective To observe the effects of dimethylarsinic acid （DMAA） on the apoptosis of gastric cancer cells. Methods The gastric cancer SGC-7901 cells were cocultured with different concentrations of DMAA, the inhibitory rate, morphological changes and cell apoptosis were detected by MTT assay, laser scanning confocal microscopy and DNA gel electrophoresis, respectively. Results The effective concentrations of DMAA inducing apoptosis were from 0.25 mmol/L to 5.0 mmol/L. Morphological changes including condensation of chromatin and fragmentation of nuclear and apoptotic bodies were observed ; Fragmentation of DNA was confirmed in electrophoretic patterns ; while DMAA at high concentrations （≥10 mmol/L DMAA） mainly caused cell necrosis. MTT assay showed that as the DMAA concentrations increased the cell viability decreased. Conclusion DMAA induces apoptosis of gastric cancer cells at low concentrations, causes cell necrosis mainly at high concentrations.
出处
《实用肿瘤杂志》
CAS
2006年第4期305-308,共4页
Journal of Practical Oncology
基金
苏州大学医学院发展基金项目(No:eel20510)
