摘要
目的：研究APBSC-TEB复合埋植于犬的腹部皮下组织，在TGF-β1和BMP的诱导下成骨，为牙周骨缺损骨修复作准备。方法：新鲜猪髂骨脱蛋白、脱钙制成TEB，浸入自犬提取的APBSC种子细胞悬液中，制备成APBSC-TEB复合物，埋植于犬腹部皮下组织，在不同时间观察成骨情况。结果：实验组术后1周APBSC细胞与移植前没有变化；术后2周，APBSC细胞壁触突向胶元纤维和成纤维细胞中伸展；术后1个月，TEB被机体吸收，代之以少量的骨小梁形成，APBSC已演变为成骨细胞。3～5个月，骨小梁增多，其间也有大量的结缔组织成分。对照组为结缔组织，没有成骨细胞形成。结论：APBSC-TEB复合移植于非骨部位，在TGF-β1和BMP的诱导下可以形成骨样组织。
Objective. For observes generate bone , we are transplanted APBSC-TEB in to dog abdomen, for the perparation transplantion bone. Method： TEB are manufactured through pig Frash ilium bone cleared off protein and calcium. TEB are immerged APBSC-liquid for get to APBSC-TEB. APBSC-TEB are bury to dogs abdomen subcutaneous tissues. for observes generate bone in the different time. Result： After operation 1 week, APBSC cell no changes in the experiment groups. After operation 2 weeks, APBSC cell-point extend to between collagenous fiber and fibroblast, APBSC cell changes to osteoblast. After operation 1 month,TEB are absorb in the dog body. there are few generate osteameba. After operation 3-5 month, there are better genrate osteameba, But there are plentiful conjunctive tissues. in the control groups, It is conjunctive tissues, no seen osteoblast. Conclusion： APBSC-TEB transplantion to not bone location, It may be generate likebone in TGF-β1 and BMP inducement.
出处
《临床口腔医学杂志》
2006年第8期454-456,共3页
Journal of Clinical Stomatology
基金
陕西省社会发展攻关计划项目基金资助(No:2004-k16-G16)
关键词
组织工程骨
外周血干细胞
转化生长因子-β1
骨形成蛋白
tissus engineering bone（TEB）
autologous periphera blood stem cell（APBSC）
transforming growth factor-β1 （TGF-β1）
bone morphogenetic protein （BMP）
