摘要
1-磷酸鞘氨醇(S1P)是具有广泛生物学效应的磷脂代谢产物,多种生长因子均能通过影响细胞鞘氨醇激酶(SphK)活性而调节S1P的生成,重组人粒细胞集落刺激因子(rhG-CSF)是一种广泛用于外周血造血干细胞动员的一种细胞因子。本研究主要了解rhG-CSF动员对供者血浆S1P含量的影响。分离供者rhG-CSF动员前和动员第5天外周血单个核细胞和血浆,用RT-PCR检测动员前后单个核细胞SphKmRNA表达情况,并应用γ-32P-ATP掺入法和SphK酶促反应检测动员前后血浆S1P变化。结果表明,供者动员前后外周血单个核细胞均表达SphKmRNA;在rhG-CSF动员前后供者血浆中均含有S1P,且S1P浓度在rhG-CSF动员前和动员后第5天无明显变化(P>0·05)。结论rhG-CSF动员对供者血浆S1P含量无明显影响。
Sphingosine 1-phosphate （SIP）, which can be impacted by different growth factors through sphingosine kinase （SphK） , is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Recombinant human granulocyte-colony-stimulating factor（rhG-CSF） have been used widely in peripheral blood stem/progenitor cell mobilization, This study was aimed to investigate the effects of rhG-CSF on SIP concentration in plasma of donors, The peripheral blood mononuclear cells and blood plasma were collected from 8 peripheral blood progenitor cell donors before mobilization and on the fifth day of mobilization with rhG-CSF. The SphK mRNA expression of blood mononuclear cells were detected by RT-PCR, The changes of SIP concentration were assayed with SphK enzyme catalyzed reaction, The results showed that both kinds blood mononuclear cells collected before and after rhG-CSF mobilization expressed SphK mRNA. The SIP concentration is low in donor＇s plasma both before and after mobilization with rhG-CSF, and there was no markedly change of Sl P concentration in plasma before and after mobilization（ P 〉 0.05 ）, In conclusion, mobilization with rhG-CSF does not impact SIP concentration in donors＇plasma.
出处
《中国实验血液学杂志》
CAS
CSCD
2006年第4期783-786,共4页
Journal of Experimental Hematology
基金
解放军总医院十五科研计划基金重点资助项目
编号01YZ05
