摘要
目的:选择αMSH作为重组毒素的导向部分,来自人类本身的血管生成素基因Ang作为毒性部分,合成具有高度特异性的重组毒素。方法:用PCR方法将MSH基因和Ang基因通过柔性Linker(Gly4Ser)2,连接成为一个基因,并定向克隆到原核表达载体pET20b中,构建了重组毒素的表达质粒。结果:MSH基因和Ang基因可以合成重组质粒pET/MSHAng。结论:通过酶切鉴定和核酸序列测定证明了重组毒素表达质粒构建的正确性。
Objective：Choosing α-MSH as the targeting part and Ang as the toxic part, a specificity recombinant toxin using the genes of α-MSH and Ang was constructed. Methods： Gene recombinant technique was used to combine α-MSH gene with toxin Ang gene and cq cloned into PET-20b plasmid. Construct recombination protein in PCR method. Results：pET/MSH-Ang could be constructed by α-MSH gene and Ang gene. Conclusion：Constructing of expression plasmid was right by restriction endonucleases and DNA sequencing.
出处
《临床肿瘤学杂志》
CAS
2006年第7期527-529,532,共4页
Chinese Clinical Oncology
