摘要
以转甜菜碱醛脱氢酶(BADH)基因水稻品系52-7的受体亲本中花8号、旱作品种开系7和陆稻白珍珠为对照,分别用含0、5、7 g?L-1NaCl的水稻专用营养液培养水稻幼苗,对转BADH基因水稻品系52-7的抗盐性及其机理进行研究.结果表明:在正常培养条件下,转基因水稻幼苗比对照品种长势旺,根系活力强,可溶性糖和叶绿素含量高,抗氧化酶SOD和POD活性高.在盐胁迫条件下,水稻幼苗生长减慢,根冠比值减小,根系活力增强;膜透性和丙二醛(MDA)含量增加,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性提高;脯氨酸和可溶性糖含量升高,叶绿素含量下降,蛋白质分解加强;且随着盐浓度的升高各指标变化幅度增加.与对照品种比较,转基因水稻幼苗在盐胁迫下的生长量和根冠比较大,电解质相对渗出率和MDA含量较低,蛋白质和叶绿素分解较少,表现出较强的抗盐性.盐胁迫下转基因水稻幼苗比对照品种具有更高的脯氨酸和可溶性糖含量以及SOD和POD活性,使其抗盐性强.
The study cultured the seedlings of ＇52-7＇,a transgenic rice line with transformed BADH gene and its BADH -- recipient parents,＇Zhonghua 8＇,＇Kaixi 7＇,a dryland variety, and ＇Baizhenzhu＇,a upland rice variety,in the specialized nutrient solutions whose NaCl contents were separately set at 0,5 and 7 g ·L^-1 to examine the salt resistance and its mechanism of ＇52-7＇ with the parents as the controls. It showed that under normal circumstances the seedlings of the transgenic rice line had stronger vigor of growth and roots,higher soluble sugar and chlorophyll contents,higher SOD and POD activities than the seedlings of the controls. Under the salt stresses the rice seedlings grew slowly, reduced their root/shoot ratios and increased the vigor of their roots;they increased their membrane permeability,MDA content and SOD and POD activities;they increased their proline and soluble sugar contents and decreased their chlorophyll content and intensified their protein breakdown;as the salt contents increased all these parameters increased differently. Under the salt stresses,the seedlings of the transgenic rice line showed high growth rate,high root-shoot ratio,low electrolyte-exudation rate,low MDA content and weak protein and chlorophyll breakdowns thus having strong salt resistance compared with the seedlings of the controls. Under the stresses the seedlings of the transgenic rice line had much higher proline and soluble sugar contents,much higher SOD and POD activities than those of the controls so that they have strong salt resistance.
出处
《西北植物学报》
CAS
CSCD
北大核心
2006年第8期1627-1632,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
河北省秦皇岛市科技局资助项目
